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Note:
alliedacademies Joint Event on
Dental Congress 2018 & World Dermatology 2018 Archives of General Internal Medicine | ISSN: 2591-7951 | Volume 2
WORLD DERMATOLOGY AND COSMETOLOGY CONGRESS
DENTISTRY AND ORAL HEALTH
&
International Conference on
Muhammad Nadhir Alkaff et al., Arch Gen Intern Med 2018, Volume 2 | DOI: 10.4066/2591-7951-C2-005
EFFECT OF HYPERGLICEMIA DURING
PREGNANCY TO FETAL TOOTH GERM
GROWTH AND DEVELOPMENT
Muhammad Nadhir Alkaff, Dwi Merry Christmarini Robin,
Nawang Lintang Clearestha and Mei Syafriadi
Jember University, Indonesia
Diabetes mellitus (DM) is a chronic metabolic disease characterized by
hyperglycemia caused by pancreatic insulin production deficiency or
ineffectiveness of the insulin produced. The objective was to determine
the effect of fetal tooth germ growth and development disturbance due to
diabetic hyperglycemia during pregnancy. About five pregnant rats were
induced by diabetes using 40 mg/kg bw streptozotocin intraperitoneally and
five pregnant normal rats as a control group. Pregnant rats with blood glucose
level ≥200 mg/dl were considered as diabetes group. Blood glucose level
was measured before, after diabetes induction and just after birth. One rat
offspring sample taken from each mother rats using simple random sampling
and euthanized on 1st day postnatal. Rat offspring right maxilla was taken
and decalcified to observe tooth germ growth and development. Each tissue
samples in paraffin-embedded tissue was cut 4 µm in thickness as many
three slides for stained using haematoxylin-eosin, Mallory’s trichrome and
insulin-like growth factor 1 (IGF-1) immunohistochemistry staining. Pregnant
rats were induced by streptozotocin showed increasing in blood glucose
with average 410±67.5 mg/dl and reduced after birth to 271±66.9 mg/dl. Rat
offspring who born from diabetic mother showed lower body weight which
is statistically significant, histologically delayed of enamel matrix formation,
delayed of tooth development stages, and reduced in tooth size compared
to control group. Therefore, seems different of IGF-1 expression in inner
enamel epithelium tooth germ between two groups. Rat offspring who born
from diabetic mother suggested had tooth germ growth and development
disturbance.
Muhammad Nadhir Alkaff has completed his bache-
lor’s degree in Faculty of Dentistry University of Jem-
ber, Indonesia and currently, he is working in Dental
Hospital University of Jember.
BIOGRAPHY