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September 16-17, 2019 | Paris, France
Dementia and Alzheimer's Disease
13
th
World Congress on
Journal of Clinical Psychiatry and Cognitive Psychology | Volume: 03
Lipid oxidation and Carotenoid supplementation
Stella Ademowo, Irundika Dias
1
, Silke De Spirt
2
, Wilhelm Stahl
2
, Helen R Griffiths
3
University of Cambridge, UK;
1
Aston University Birmingham, UK;
2
Heinrich-Heine-Universität Düsseldorf, Germany;
3
University of Surrey, UK
O
xidative stress has been considered as important in
the pathogenesis of Alzheimer’s disease, AD. Post-
mortem investigations of affected brain regions in AD
have shown the accumulation of oxidative damage to
protein, DNA and lipids. Carotenoids have the ability to
quench singlet oxygen and scavenge other reactive oxygen
species (ROS) without being consumed in the process. We
have previously shown increased concentrations of the
novel oxidized phospholipid biomarker, 1-palmitoyl-2(5-
oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC) and
lower carotenoid plasma concentrations in AD. POVPC
was analysed using electrospray ionisation tandem mass
spectrometry (MS) with multiple reaction monitoring
(MRM), 8-isoprostane (IsoP) was measured by ELISA and
ferric reducing antioxidant potential (FRAP) was measured
by a colorimetric assay in AD patients and healthy age-
matched control subjects. The developed MRM-MS
method was used to analyse POVPC as a measure of
peroxidative damage to phospholipids in serum. Using
this method, the peroxidised phospholipid POVPC was
found to be higher in AD patients and was correlated with
cognitive performance but not reduced by carotenoid
supplementation. We also investigated the protective role
for carotenoids against mitochondrial dysfunction induced
by POVPC (1-20µM) in differentiated (d)SH-SY5Y neuronal
cells. POVPC, lutein (0.1-1µM) and zeaxanthin (0.05-
5µM) were recovered in dSH-SY5Y cells after 24 hours of
treatment. Glutathione (GSH) levels, mitosox oxidation
and mitochondrial function were analysed in cells treated
with POVPC (1-20µM) and co-incubation with carotenoids
(lutein and zeaxanthin). We found pathophysiological
concentration-induced damage can be protected with
appropriate dose of carotenoid.
The talk will highlight some of our main findings on the
effects of carotenoid supplementation on lipid oxidation.
e:
osa25@cam.ac.uk