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Journal of Microbiology: Current Research | Volume 2
November 01-02, 2018 | London, UK
7
th
European
Clinical Microbiology Congress
4
th
International Conference on
Ophthalmology and Eye Disorder
Joint Event
&
Analysis of thoracic proteins of female
Aedes togoi, Anopheles lesteri
and
Anopheles paraliae
,
responsible for nocturnally subperiodic
Brugia malayi
infection
Watcharatip Dedkhad, Onrapak Reamtong, Wanpen Chaicumpa, Chayanit Hempolchom
and
Atiporn Saeung
Chiang Mai university, Thailand
B
rugia malayi
is a mosquito-borne filarial nematodes
causing human filariasis lymphatic (LF). Understanding
the host responding to
B. malayi
would be useful to prevent
the transmission of the disease. The ability of pathogen
transmission is depend on the relationship between host and
parasite that occurring in the thoracic muscles. However, little is
known about mosquito proteins responding during developing
processes. We aim to characterize and compare the proteomic
profiles of the thoracic proteins of the three mosquito species
responsible for nocturnally subperiodic
B. malayi
infections.
Highly susceptible (
Aedes togoi, Anopheles lesteri
) as well as
lowsusceptible (
Anopheles paraliae
) filariasis vectorswere used
in this study. The thoraces of
B. malayi
-infected mosquitoes
(test group) and uninfected blood meals (control group) of
each mosquito species were collected at 96 hours post blood
meal. The SDS-PAGE-separated-protein profiles of
B. malayi
-
infected
Ae. togoi, An. lesteri
and
An. paraliae
showed at least
10, 9 and 8 major protein bands, respectively, whereas 6 major
protein bands were found in the control groups. Nano-liquid
chromatography mass spectrometry (nanoLC-MS/MS) revealed
22, 9 and 12 previously known proteins in
B. malayi
-infected
Ae. togoi, An. lesteri
and
An. paraliae
, respectively. Of interest,
peroxiredoxin 5, thioredoxin and superoxide dismutase, were
expressed only in
B. malayi
-highly susceptible
Ae. togoi
and
An. lesteri
. This is the first study provides the data on thoracic
protein profile responding during
B. malayi
development and
demonstrates that antioxidant and detoxifying proteins might
play important role and/or provide favorable environment in
facilitating further development of the
B. malayi
microfilariae
to the human infective stage in the vectors.
Speaker Biography
Watcharatip Dedkhad is a PhD student at the Department of Parasitology, Faculty of
Medicine, Chiang Mai University, Thailand. She has published 4 papers in reputed journals.
e:
wd.dedkhad@gmail.comWatcharatip Dedkhad et al., Clinical Microbiology and Eye 2018, Volume 2
DOI: 10.4066/2591-8036-C1-003