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June 12-13, 2019 | Edinburgh, Scotland
8
th
European Clinical Microbiology and Immunology Congress
&
3
rd
World congress on Biotechnology
Joint Event
Microbiology: Current Research | Volume: 3 | ISSN: 2591-8036
Interaction between probiotics and skin pathogens within the host
Duaa S Al-Dulaimy, Julian Marchesi
and
Eshwar Mahenthiralingam
Cardiff University, UK
T
he skin is an ecosystem which frequently interconnects
with the outer environment and colonized with a vast
number of different microorganisms. These microbial
groups are associated with human health and disease.
Skin and soft tissue infections (SSTIs) are the infections
caused as a result of the microbial invasion of the skin
layers and underlying soft tissues. They vary from mild to
severe infections. The prolonged appliance of antibiotics
can expand the incidence of antibiotic resistance. This has
led to the necessity to find a safe long-term alternative
treatment for infectious diseases. Many studies have
exposed the promising advantages of probiotics in both
prevention and treatment of diseases. Probiotic bacteria
have many valuable properties to repress the growth
of pathogenic microorganisms. Members of the genus
Lactobacillus
are one of the most common probiotics used
in fermented and non-fermented dairy products. Animal
studies were successfully demonstrated in using the wax
moth larvae
Galleria mellonella
as a model to investigate
host-pathogen interactions. This project aimed to explore
both the
in vitro
antagonistic activity and
in vivo
protective
effect of food isolated
Lactobacillus
species against two
of the major causes of skin infections:
Staphylococcus
aureus
,
Streptococcus pyogenes
. Pathogenic bacteria
were isolated from skin infections’ patients.
Lactobacillus
species were isolated from fermented food products.
Bacterial biodiversity of food samples was evaluated
by culture-independent method (16S rRNA gene meta-
analysis). Antibacterial activity of
Lactobacillus
on the
pathogens was assessed by an overlay assay. To determine
the numbers of both
Lactobacillus
and pathogenic
isolates, several serial dilutions of bacterial washed cells
were injected inside the larvae individually in triplicates.
To evaluate the therapeutic potential of
Lactobacillus
against the pathogens and depending on the larval survival
percentages, two doses of
Lactobacillus
bacterial cells
were injected in the larvae after the injection of several
doses of each pathogen. Two
Lactobacillus
species:
Lb.
delbrueckii
and
Lb. plantarum
were isolated from food
samples (yogurt and olives) inoculated in MRS broth and
incubated under anaerobic conditions. However, culture-
independent method of these samples inoculated in the
same medium and incubated under the same conditions
showed an abundance of 0.8% for the first species and no
abundance for the second species in the extracted genomic
DNA. All lactobacilli revealed the maximum antagonism
after 72h under anaerobic conditions. Injection of both
Lactobacillus
species in a dose of 103 – 104 CFU/larvae
showed 80% - 100% larval survival.
Strept
.
pyogenes
was
more virulent to the larvae than
Staph aureus
. When
compared with the control groups, low dose of one of the
Lactobacillus
species has a protective activity against the
infection caused by
Strept pyogenes
. It can be concluded
that food lactobacilli have an adequate therapeutic
potency against skin pathogens used in the study.
Speaker Biography
Duaa S Al-Dulaimy is currently pursuing her PhD in Cardiff University,
UK. She has completed her BSc degree from Department of Biology/
School of Biosciences, Mustansiriyah University, Iraq and her MSc
degree from the same department. She has published several papers
in reputable journals and has been working as a lecturer for more than
ten years teaching the undergraduate students of medical microbiology
and biotechnology. And, she has supervised several undergraduate
students.
e:
Al-Dulaimyds@cardiff.ac.ukDuaa S Al-Dulaimy et al., Microbiol Curr Res, Volume 3
ISSN: 2591-8036