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June 12-13, 2019 | Edinburgh, Scotland
8th European Clinical Microbiology and Immunology Congress
&
3rd World congress on Biotechnology
Joint Event
Microbiology: Current Research | Volume: 3 | ISSN: 2591-8036
Switches, thresholds and flux signals in the control of central metabolism’s architecture
in Escherichia coli
Mansi El-Mansi
University of Africa, Nigeria
T he ability of microorganisms to reconfigure the
topology of central metabolism from acetogenic
to gluconeogenic architecture is central to successful
adaptation and in turn, survival, as the environment
changes from “feast” to “famine”. Based on flux analysis,
measurements of enzymic activity of isocitrate lyase and
its m-RNA transcripts, the author proposes that the central
metabolic pathways of Escherichia coli are bicyclic in
nature and that the organism’s ability to switch from one
cycle to another is controlled by the “diauxic switch”, which
is turned “on” or “off” in response to a drop-in flux to ATP
to a critical thresh hold as growth rate-diminishes from
μmax to ≤0.43h-1. The shortfall in ATP supply is redressed
by increasing flux through succinyl CoA synthetase, which
under these circumstances, necessitates the operation of
the glyoxylate bypass for the provision of succinyl CoA.
Uniquely, however, yet in complete harmony with the
hypothesis presented, the glycerol phenotype does not
appear to employ the “diauxic-switch”, as glycerol affords
direct entry into the two glycolytic SLP-ATP generating
reactions, thus maintain the level of ATP above the critical
threshold required for the activation of the diauxic-switch.
Speaker Biography
Mansi El- Mansi is a PhD graduate in microbial biochemistry and
molecular enzymology, University College of Wales, Aberystwyth, UK
(1982). Immediatelyaftergraduating,he joined DrHarryHolms research
group at the University of Glasgow, department of biochemistry; under
the leadership of professor Martin Smellie. Such a happy association
continued for the best part of a decade in which many discoveries
were made on the control of isocitrate dehydrogenase activities and
the expression of the glyoxylate bypass operon. During the course of
his career at Glasgow University (9 years) and in Edinburgh (17 years)
as well as at Sharda University, India (3 years) and more recently at
Elizade University and University of Africa, Nigeria. He has had many
notable achievements in academic and applied research. In addition to
elucidating the signal, which triggers the expression of the ace operon
in vivo in preparation for the switch of central metabolism’s topology
from acetogenic to gluconeogenic architecture.
e:
Mansi El-Mansi, Microbiol Curr Res, Volume 3
ISSN: 2591-8036