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Chromatography 2018 & Satellite 2018 Journal of Chemical Technology and Applications| Volume 2
&
CHROMATOGRAPHY AND SEPARATION SCIENCE
World Congress on
SATELLITE AND SPACE MISSIONS
International Conference and Exhibition on
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Lokesh Kumar Gupta, J Chem Tech App 2018, Volume 2
MODERNIZATION IN LIQUID
CHROMATOGRAPHY: MANUAL TO GREEN
SEPARATIONS
From decades chromatography has been accepted as a key analytical tool
for understanding and characterization of molecules in chemistry, the level
of world-wide research activity in this field promises that these capabilities
will continue to improve, rapidly. Over the period of time sensitivity of chroma-
tography tools have been improved to work at micro level and in more depth,
ultimately become a faster research tool; such tools now being used as pro-
cess analytical tools (PAT) giving online understanding of characteristics of
a molecule during research/development and in production. Same time their
utilization has widen up by coupling these tools with mass spectrometry and
NMR etc e.g. (LC-MS-MS, LC-NMR ...etc.) Primary Liquid chromatography
was defined in the early 1900s by the work of the Russian botanist, Mikhail
S. Tswett. Today, liquid chromatography, in its various forms, has become one
of the most powerful tools in analytical chemistry. The acronym HPLC, was
coined in 1970, it was equipped with a pump (~35 bar). The early 1970s saw
a tremendous leap in technology with wearing pressure >400 bar and incorpo-
rated improved injectors, detectors, and columns. HPLC really began to take
hold in the mid-to late-1970s. With continued advances in performance during
this time the acronym HPLC remained the same, but the name was changed
to high performance liquid chromatography (HPLC). HPLC is now one of the
most powerful tools in analytical chemistry. It has the ability to separate, iden-
tify, and quantitate the compounds that are present in any sample it can be dis-
solved in a liquid. Today, compounds in trace concentrations as low as parts
per trillion (ppt) may easily be identified. HPLC now has become the backbone
for the analysis of pharmaceuticals, food, nutraceuticals, cosmetics, environ-
mental matrices, forensic samples & industrial chemicals. In the starting of
21st century, further advancement in HPLC came out in terms of UPLC (Ultra
Performance…..) (>1000 bar), faster separation with improved resolution &
speed. This technology represented a candid revolution and leadership with
excitement and new courage in analytical science with confidence and reli-
able/reproducible results. One step ahead towards green chemistry, primary
concern for elimination or major reduction in the use of organic solvents in
chromatography opened the doors to another technology: Supercritical CO2
extraction, where major part of the mobile phase is CO2 (non-toxic, non-flam-
mable and physiologically compatible). Compared to other techniques, use of
SFE/SFC, the compounds are cleaner, efficiency is higher, selectivity is better,
yield is higher and costs are lower apart from being the Green Technology, it is
leading separation/purification technology. In this review, I had evaluated and
focused on advancement and updates in LC field, with respect to technology
update & applications.
Biography
Lokesh Kumar Gupta has completed his PhD at
the age of 25 by researching in University of Del-
hi and Ch. CS University Meerut, India. He is an
analytical research scientist and serving as Chief
Manager of Analytical R&D team with TEVA API (a
world leader in generic pharmaceuticals) in India,
focusing on analytical research/methods develop-
ment, spectroscopic characterization of organic
pharmaceuticals, technology validation, impurity
isolation & characterization etc. He also works for
ensuring the laboratory compliance across all sites
of TAPI, around the globe. He is participating and
discussing his commended research in several na­
tional/international seminars/conferences. He had
published more than 42 papers in peer reviewed
reputed journals of chemistry & spectroscopy and
serving as an eminent referee for several journals
of international repute.
Lokesh Kumar Gupta
TEVA API, India