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Page 37
Cell and Gene Therapy 2018 & Clinical Microbiology Congress 2018
Biomedical Research
|
ISSN: 0976-1683
|
Volume 29
S e p t e m b e r 1 0 - 1 1 , 2 0 1 8 | D u b l i n , I r e l a n d
allied
academies
Joint Event on
CLINICAL AND MEDICAL MICROBIOLOGY
CELL AND GENE THERAPY
&
World Congress on
International Conference on
Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C3-008
EFFECT OF HUMAN UMBILICAL CORD BLOOD-DERIVED MONONUCLEAR
CELLS ON DIABETIC NEPHROPATHY IN RATS
Nahla E El-ashmawy, Eman G Khedr,
Hoda A El-bahrawy
and
Shimaa A El-berashy
Tanta University, Egypt
D
iabetic nephropathy (dn) is damage to the kidney which can lead to chronic renal failure, eventually requiring dialysis. Diabetes
mellitus is the most common cause of adult kidney failure worldwide in the developed world. The current work was designed to
elucidate the effect of mononuclear cells (mncs) injection on reverse dn in rats exposed to streptozotocin (stz) injection compared to
metformin as a known hypoglycemic drug, 40 male rats were divided equally into four groups; normal control group, diabetic control
group, mncs group were diabetic rats treated with mncs (30×106 mncs/rat once iv dose) in the tail vein of the rat, and metformin
group were diabetic rats treated with metformin (100 mg/kg orally daily dose) for four weeks. The results indicated an improvement
effect of mncs and metformin on stz-induced dn in rats, which was evidenced by significant decrease in urinary albumin/creatinine
ratio, n-acetyl-β-d-glucosaminidase (nag), urinary kidney injury molecule- 1 (kim-1), serum urea, serum creatinine and fasting blood
glucose and significant increase in c- peptide level, compared to diabetic control group. Additionally, mncs treated group exhibited
pronounced effects in all previous parameters compared to metformin treated group. It is proved that mncs treatment was superior
to metformin in controlling hyperglycemia and improving renal function in diabetic rats.