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Page 28
Cell and Gene Therapy 2018 & Clinical Microbiology Congress 2018
Biomedical Research
|
ISSN: 0976-1683
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Volume 29
S e p t e m b e r 1 0 - 1 1 , 2 0 1 8 | D u b l i n , I r e l a n d
allied
academies
Joint Event on
CLINICAL AND MEDICAL MICROBIOLOGY
CELL AND GENE THERAPY
&
World Congress on
International Conference on
Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C3-008
REGENERAGE SYSTEM: THERAPEUTIC EFFECTS OF COMBINATORIAL
BIOLOGICS (MRNA AND ALLOGENIC MSCS) WITH A SPINAL CORD
STIMULATION SYSTEM ON A PATIENT WITH SPINAL CORD SECTION
Joel I Osorio
Westhill University School of Medicine, Mexico
A
s it has been previously demonstrated that co-electroporation of Xenopus laevis frog oocytes with normal cells and cancerous
cell lines in-duces the expression of pluripotency markers, and in experimental murine model studies that mRNA extract
(Bioquantine® purified from in-tra- and extra-oocyte liquid phases of electroporated oocytes) showed potential as a treatment for a
wide range of conditions as Squint, Spinal Cord Injury (SCI) and Cerebral Palsy among others. The current study observed beneficial
changes with Bioquantine® administration in a patient with a severe SCI. Pluripotent stem cells have therapeutic and regenerative
potential in clinical situations CNS disorders even cancer. One method of reprogramming somatic cells into pluripotent stem cells
is to expose them to extracts prepared from Xenopus laevis oocytes. We showed previously that coelectroporation of Xenopus
laevis frog oocytes; with normal cells and cancerous cells lines, induces expression of markers of pluripotency. We also observed
ther-apeutic effects of treatment with a purified extract (Bioquantine) of intra- and extra-oocyte liquid phases derived from
electroporated X laevis oocytes, on experimentally induced pathologies including murine models of melanoma, traumatic brain
injury, and experimental skin wrinkling induced by squalene-monohydroperoxide. The positive human findings for spinal cord injury,
and cerebral palsy with the results from previous animal studies with experimental models of traumatic brain injury, respectively.
Because of ethical reasons, legal restrictions, and a limited number of patients, we were able to treat only a very small number of
patients. These results indicate that Bioquantine® may be safe and well tolerated for use in humans and deserves further study in
a range of degenerative disorders. We propose that the mechanism of action of Bioquantine® in these various diseases derives
from its unique pharmacology and combinatorial reprogramming properties. In conclusion, these preliminary findings suggest
that Bioquantine is safe and well tolerated on patients with Cerebral Palsy and-Spinal Cord Injury, among others. In addition to
the regenerative therapy and due to the patient condition, we decided to include the Restore-Sensor SureScan. Based on the of
electrical stimulation for rehabilitation and regeneration after spinal cord injury published by Hamid and MacEwan 8-9 , we designed
an improved delivery method for the
in-situ
application of MSCs and Bioquantine® in combination with the RestoreSensor®
SureScan®. To the present day the patient who suffered a total section of spinal cord at T12-L1 shows an improvement in sensitivity,
strength in striated muscle and smooth muscle connection, 11 months after the first therapy of cell regeneration and three month
after the placement of RestoreSensor® at the level of the lesion, the patient with a complete medullary section shows an evident
improvement on his therapy of physical rehabilitation on crawling from front to back by himself and standing on his feet for the first
time and showing a progressively important functionality on the gluteal and legs sensitivity.