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S e p t e m b e r 0 6 - 0 7 , 2 0 1 8 | B a n g k o k , T h a i l a n d
Biotechnology Congress 2018 & Emerging Materials 2018
Note:
Page 34
allied
academies
EMERGING MATERIALS AND NANOTECHNOLOGY
BIOTECHNOLOGY
&
Annual Congress on
Global Congress on
Joint Event on
Biomedical Research
|
ISSN: 0976-1683
|
Volume 29
Ashok Kumar Srivastava, Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C4-009
PLANT CELL CULTIVATION OF
A INDICA
IN BIOREACTOR FOR MASS PRODUCTION
OF AZADIRACHTIN
P
resently bio-pesticide (azadirachtin) is produced by solvent extraction
of
Azadirachta indica
(Neem) seeds. However, this protocol features low
variable yield and severe contamination problems.
In-vitro
plant cell cultivation
in bioreactors can serve as an alternate for mass production of azadirachtin.
Successful attempt was made to initiate callus from the seed kernel of high
yielding variety of
Azadirachta indica
. This was followed by development
of shake flask suspension culture under statistically optimized culture
conditions for maximum growth and azadirachtin production. The biomass
and azadirachtin yield enhancement strategies were then applied (which
included addition of elicitors, precursors, growth factors and permeabilizing
agents) to enhance azadirachtin production. Batch growth and azadirachtin
production kinetics of the cell suspension culturewas there after established in
a stirred tank bioreactor. Engineering optimization was then attempted where
in a mathematical model was developed using the batch kinetic data. This
served as an excellent tool to understand the culture behaviour under different
cultivation conditions and facilitate the design of an appropriate feeding
strategy(ies) in fed-batch/continuous mode(s) of cultivation. A pre-optimized
computer simulated continuous cultivation strategy with cell retention was
experimentally implemented which featured high biomass and azadirachtin
production from
A indica
cells in the bioreactor. The experimental verification
resulted in a biomass accumulation of 61.4 g/L and azadirachtin production
of 751.9 mg/L with 14% release of azadirachtin (105.0 mg/L) in the culture
medium. This was significantly higher than the azadirachtin production from
seeds from natural plant of neem.
Biography
Ashok Kumar Srivastava has received his PhD de-
gree from the McGill University, Montreal in 1990.
He has 40 years of industrial research teaching ex-
perience in Biochemical Engineering and Biotech-
nology. He has 110 international journal papers,
154 international/national presentations and two
patents to his credit. He has supervised 16 PhD
(five continuing) and 73 master’s theses. His ma-
jor interest is in modelling simulation, optimization
and control of bioprocesses, microbial/plant cell/
hairy root cultivations for important metabolite
production (bio/copolymer production, podophyl-
lotoxin, azadirachtin, ajmalicine, shikimic acid pro-
duction etc) and novel bioreactor development.
ashokks@dbeb.iitd.ac.inAshok Kumar Srivastava
Indian Institute of Technology, India