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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
In vitro
anti-leishmanial activity of
Artemisia dracunculus
and
Heracleum persicum
extracts in comparison
with glucantime
Batool Sadeghi-Nejad
1
, Shahram Khademvatan
2
, Alborz Eskandari
3
and
Sedigheh Yusef Naanaie
4
1
Abadan School of Medical Sciences, Abadan, Iran
2
Urmia University of Medical Sciences, Iran
3
Jundishapur University of Medical Sciences, Iran
4
The Agricultural and Natural of Resources Center, Iran
Background & Objectives:
Cutaneous leishmaniasis (CL) is
one of the most common parasitic diseases. It is one of the
major public health in developing countries and throughout
the world. Pentavalent antimonial compounds like pentostam
and glucantime has been used to treat CL for the last 50 years.
The use of these compounds has some limitations such as long
duration of treatment, high expenses of drugs, and methods
of drug use which are intradermal and intramuscular injection.
Beside these, lack of response to the treatment in 10-15% of
cases and toxic effects on heart, liver, and kidneys are other
possible side effects. Hence, the objective of the present survey
was to state the antileishmanial activity of two herbal medicine
(
Artemisia dracunculus and Heracleum persicum
) extracts were
evaluated against
Leishmania
major and Leishmania infantum
using colorimetric MTT (2-(4,5-dimethyl-2-thiazolyl)-3,5-
diphenyl-2H-tetrazolium bromide) assay and compared to the
glucantime as a reference.
Materials & Methods:
The leaves extracts of selected plants
were obtained by maceration. The
in vitro
assays were carried
out on L. major and L. infantum using colorimetric MTT assay
in comparison with glucantime. The concentration-response
curves tested extracts and glucantime solutions were designed
and IC50 values were located.
Results:
Anti-Leishmina effects of
A.
dracunculus and H.
persicum
) on L. major and L. infantum promastigote were
revealed with 50% inhibitory concentration (IC50) values of
49.67 and 42.23 mg ml-1 for
A. dracunculus
, 81.15 and 73.17
mg mg ml-1 for H. persicum. In comparison with the standard
drug, glucantime had IC50 value of 40.2 mg ml-1 for L. major
and 18.5 mg ml-1 for L. infantum promastigote after 72 hours
incubation respectively.
Conclusion:
These results revealed that compounds from
Satureja khuzestanica
and
Heracleum persicum
have anti-
leishmania properties that necessary to survey the effects of
these extracts on
leishmania
genus in animal models in future.
Speaker Biography
Batool Sadeghi-Nejad is working at Abadan School of Medical Sciences, Abadan, Iran.
She has published many research papers.
e:
batsad4@yahoo.com