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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
Isolation, identification and antibiotic susceptibility profiles of bacterial strains isolated from supragingival
plaque of periodontal patients at dental service and training center of Addis Ababa University
Solomon Gizaw
Addis Ababa University College of Health Sciences, Ethiopia
P
eriodontal disease is the common oral problem of human
being that cause permanent tooth lose. In our study, we
have determined the periodontal bacterial pathogens with
their susceptibility profiles and the potential risk factors for
the disease. Poor oral hygiene practice increases biofilm/
plaque formation on the teeth. Dental Plaque formed on
the supragingival area can harbor different pathogens that
can cause periodontal disease. Microbial infections of the
periodontal tissues lead to the destruction of the alveolar bone
and cementum accompanied by mobility of teeth. A cross
sectional study was conducted from April 2015 to June 2015.
A total of 384 clinically confirmed periodontal patients were
recruited for the study. 52.9% of them were males and the
median age was 35 years. Culture positives were characterized
by using standard biochemical tests and API ID Microsystems
(bioMérieux, France). Antimicrobial susceptibility test was
performed using CLSI and EUCAST 2015. Tested drugs were
selected using these guidelines. Microbiological investigations
of samples lead to the isolation 459 different types of bacterial
strains. The most frequently isolated species were Gram-
positive facultative anaerobes and anaerobic gram-negative
rods.
Candidia albicans
was also seen in 1.9% (n=9). The
antibiotic susceptibility patterns of Gram-positive facultative
anaerobes mainly
Streptococcus
species
show high resistance
rates to ciprofloxacin 20.5% and amoxacillin 20.3%. Drug
resistance for a single drugwas seen in 56.1%of the isolates and
20.6% of the isolates was susceptible for all the drugs tested.
MDR=resistance for ≥2 drugs were seen in 34.7%of the isolates.
Speaker Biography
Solomon Gizaw is working in the Department of Medical Microbiology, Immunology
and Parasitology, Faculty of Medicine, Addis Ababa University, College of Health
Sciences.
e:
soloasme2000@gmail.com