21 / 21
Page 67
allied
academies
Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
Aflatoxins have been emerged as a serious threat to food
safety and quality assurance. A variety of fungal species
i.e., multiple strains of
Aspergillus flavus, Aspergillus niger,
Fusarium oxysporum, Alternaria alternata
have been
reported as aflatoxigenic. Main objective of this study was
to evaluate the genes responsible for aflatoxins production
in the fungal strains isolated from rice grains being stored
in warehouses of district Lahore, Pakistan. Total five (05)
representative samples of rice grains were obtained
and analysed for the fungal microflora. Isolation and
identification of fungal strains were initially done based on
morphological characters, later confirmed by using universal
primers of internally transcribed spacers regions (ITS-1 and
ITS-4). Eleven (11) fungal strains were identified based on
phylogenetic analyses. Potential to produce afaltoxins was
checked by using a set of specific primers including genes
of aflR,
nor1, omt1, ver1
. Four (04) out of total eleven (11)
strains showed the potential to produce aflatoxins. This
might be due to humidified environment of warehouse that
is helpful for the rapid growth of fungal strains. Research for
the root causes for the growth of aflatoxigenic fungal strains
in warehouses is continued.
e:
warraych@gmail.comMolecular analysis of aflatoxigenic fungal strains on rice grains
Sohaib Afzaal, Shinawar Waseem Ali and Usman Hameed
University of the Punjab, Pakistan