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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
Statement of the problem:
In nature, plants are subject to
several diseases due to the presence of pathogens. Fungal
diseases are commonly controlled through the use of
pesticides which have resulted in clinical and environmental
damages. The use of beneficial bacteria may promote diverse
beneficial function in intensive agriculture such as biological
control, where bacteria can exhibit antagonistic interactions
to compete for space and nutrients in their habitat. The best
known antagonistic bacteria are
Enterococcus, Lactococcus,
Streptomyces, Bacillus, Pseudomonas, Klebsiella, Escherichia,
and
Burkholderia
due to their potential to produce inhibitory
substances such as broad-spectrumantibiotics, organic acids,
siderophores, antifungal and bacteriocins. Our study model,
Pseudomonas
sp. EMM-1, is a Gram-negative bacterium
isolated from contaminated soil highly competitive due to
the production of one or more inhibitory substances. Its
antimicrobial activity was demonstrated against diverse
beneficial and pathogenic microorganisms including the
genera
Bradyrhizobium, Azotobacter, Staphylococcus,
Streptococcus, Klebsiella
and
Burkholderia
; as well as the
phytopathogenic fungi
Pantoea
and
Fusarium
.
Methodology & Theoretical Orientation:
Bacterial
competition is mainly evaluated by double-layer agar and
simultaneous inhibition assays. In this work the double-layer
agar methodology was performed to evaluate the ability
of Pseudomonas sp. EMM-1 to antagonize diverse fungi
isolated from soil and plants with fungal diseases such as
Aspergillum
and
Fusarium
.
Conclusion & Significance:
The results of inhibition assays
suggest that
Pseudomonas
sp. EMM-1 is able to produce
metabolites that inhibit the growth of diverse fungi, leading
us to assume its potential as biocontrol agent.
e:
kathy_cl_3@hotmail.comAntagonism of
Pseudomonas
sp. EMM-1 and its potential as bio-control agent
Catherine Cesa-Luna, Joel de la Cruz-Enríquez, Ana Laura Hernández-Tenorio, Fernando Xicale-Nava, Yolanda Elizabeth Morales-García, Rocío Pérez-y-
Terrón, Antonino Báez-Rogelio, Jesús Muñoz-Rojas
and
Verónica Quintero-Hernández
Universidad Autó noma de Puebla, Mexico