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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
Background:
Bacteria live either independently as planktonic
cells or in organized surface associated colonies called
as biofilms. Biofilms play an important role in increased
pathogenesis of bacteria and it is assumed that, motility is
one of the contributing factor towards biofilm initiation.
Aims:
This study was planned to identify the role of flagella
in biofilm formation by constructing flagellated (wild type)
and physically disrupted variants (non-motile).
Methods:
Total 10 clinical bacterial strains were screened.
Based on morphological variation and motility, only two
highly resistant trains were characterized biochemically,
physiologically and genetically. Biofilm formation capacity of
strains was analyzed using three methods including Congo
red assay, test tube assay and liquid-interface coverslip assay.
Afterwards, flagellar disintegration was induced by blending
and centrifugation for 5, 10 and 15 minutes.
Results:
Our results showed these strains as
Bacillus cereus
and
Yersinia enterocolitica
identified by 16S rRNA sequencing.
Both strains produced significant biofilm by all three above
mentioned methods. A motility test of these blended
variants showed partial leading to completely diminished
motility with increased blending time. The significant loss
in biofilm formation after 15 minutes of blending confirmed
the important contribution of flagella to the initiation of
biofilm formation. This biofilm defect observed in flagella
paralyzed/minus variants presumably may be due to defects
in attachments to surface at early stages.
Conclusion:
This study indicated that flagellar motility is
crucial initially for surface attachment and subsequently for
biofilm formation.
e:
iramliaq@hotmail.comFlagellar Motility plays critical role in biofilm formation of
Bacillus cereus
and
Yersinia enterocolitica
Iram Liaqat
Govt. College University, Pakistan