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Analytical Chemistry 2018
Journal of Chemical Technology and Applications
|
Volume 2
ANALYTICAL CHEMISTRY
International Conference on
Wael Abu Dayyih et al., J Chem Tech App 2018, Volume 2
DETERMINATION ATORVASTATIN
PHARMACOKINETIC PARAMETERS IN
RATS BY LC/MS-MS WITH TRADITIONAL
LICORICE BEVERAGE
Wael Abu Dayyih
and
Eyad Mallah
University of Petra, Jordan
A
torvastatin is a synthetic second generation3-hydroxy-3- methyl-glu-
taryl-coenzyme (HMG-CoA) reductase inhibitor which lowers plasma
cholesterol levels by inhibiting the HMG-CoA reductase that catalyzes the
conversion of HMG-CoA to mevalonate, an early and rate-limiting step in cho-
lesterol biosynthesis in the liver. Atorvastatin is metabolized in the body by
cytochrome P450 3A4 enzyme system (abbreviated CYP3A4). Atorvastatin is
widely used in many countries in the treatment of severe familial or non-fa-
milial hypercholesterolemia. The objective of this randomized controlled
study was firstly, developing and validating a simple and simultaneous LC/
MS method to quantify Atorvastatin in rat serum. Secondly, investigating the
effects of Liquorice fresh beverage on the pharmacokinetics of Atorvastatin
in serum rats. Atorvastatin was determined in rat’s plasma using high reso-
lution, sensitivity and validated Liquid Chromatography–Mass Spectrometry
(LC-MS); The mobile phase was (75% methanol, 25% of 0.2% Formic acid in
water), ACE 5 C8 Column (50 X 2.1 mm, 5µ), 1.0 ml/min flow rate, 5 micro-
liters Auto-sampler injection volume, and Glimepiride as an internal stan-
dard. The analytical method was linear with acceptable recovery, precision
and accuracy. A significant (P<0.05) effect of single dose of Liquorice after 2
hours from administration of Atorvastatin, and significant effect of repeated
doses of Liquorice after 6 hours from administration of Atorvastatin. There
was no significant effect (P>0.05) of single dose Liquorice juice on Atorvas-
tatin C-max (29.847 ng/ml), and repeated doses of Liquorice the C-max was
(40.113 ng/ml) which is also considered as a non- significant effect (P>0.05)
We conclude that the effects of Liquorice beverage on Atorvastatin varies
between single and repeated doses and this can be a result and could affect
Atorvastatin pharmacokinetics.
Keywords:
Licorice, Atorvastatin, LC/MS. Rat Plasma, Validation, Pharmaco-
kinetic.
Wael Abu Dayyih is a PhD holder and currently with the
Department of Pharmaceutical Medicinal Chemistry and
Pharmacognosy, Faculty of Pharmacy and Medical Sci-
ences-University of Petra, Jordan. His reseach interests
include HPLC; Pioglitazone; Sucralose; Pharmacokinetic;
Interaction.
wabudayyih@uop.edu.joBIOGRAPHY