zoology-microbiology-2017-posters-accepted-abstracts

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J Parasit Dis Diagn Ther 2017 | Volume 2 Issue 4

International Conference on

Zoology, Microbiology & Medical Parasitology

October 30-November 01, 2017 | Chicago, USA

Introduction:

Diplostomid metacercariae

inhabit freshwater

fish species as the second intermediate hosts. These parasites

have been found in the eye lens, the retina, vitreous humor

and the nervous system of freshwater fish. The classification

of these parasitic stages to the species level using only

morphology is often difficult and ambiguous. The use of

molecular techniques has allowed links to be elucidated

using various developmental stages of these parasites. The

aim of this study was to provide a summative report on the

phylogenetic tree by applying molecular biology techniques

to the investigation of larval diplostomid parasites.

Materials and Methods:

Diplostomid metacercariae

were

preserved in 70% ethanol prior to DNA extractions using

Qiagen kit. Standard techniques for amplification of rRNA

region were followed. The DNA amplicons were sent to

inqaba Biotech laboratory for sequencing and phylogenetic

trees generated using software programs.

Results:

The amplicons of these diplostomids had band

sizes of 500 base pairs. The amplicons contained only partial

regions (ITS-2). The parasitic species 28S rDNA genomic

region was successfully amplified.

Conclusion:

The application of molecular techniques on

digenetic trematodes seems very promising and may yield

great potential in future descriptions of morphologically

similar parasitic species.

;

Report on the phylogenetic studies of diplostomatids parasites

Mitonga M.M.A, Moema E.B.E, King P.H

and

Rakgole J.N

Sefako Makgatho Health Sciences University, South Africa