world-biochem-regenerative-medicine-2019-scientifictracks

Note:

World Biochem 2019 & Regenerative Medicine 2019

Journal of Genetics and Molecular Biology | Volume 3

Page 45

OF EXCELLENCE

IN INTERNATIONAL

MEETINGS

YEARS

March 25-26, 2019 | Amsterdam, Netherlands

BIOCHEMISTRY AND ENZYMOLOGY

World Congress on

TISSUE ENGINEERING AND REGENERATIVE MEDICINE, STEM CELL RESEARCH

Global Conference on

Joint Event on

VASCULARIZATION OF A BONE MARROWMODEL

Kübrah Keskin, Sieber S, Marx U, Lauster R

and

Rosowski M

Technische Universität Berlin, Germany

he bone marrow is, as a harbour of the endosteal and perivascular niche of haematopoietic stem and progen-

itor cells (HSPCs), an important organ of the human body and has a tremendous role in regenerative medi-

cine. Sieber et al. (2017) mimicked the endosteal niche by developing a dynamic bone marrow model harbour-

ing HSPCs in co-culture with Mesenchymal Stromal Cells (MSCs) for up to eight weeks in a hydroxyapatite coat-

ed zirconium oxide-based ceramic. The cultivation of the 3D construct is realized within the “Multi-organ-chip”

(MOC) developed at our chair. The MOC is a microfluidic device consisting of a circular channel system which

connects two wells to cultivate organoids. To additionally mimic the perivascular niche, vascular structures must

be added to the model. HUVECs, in co-culture with MSCs, elongate and form a primitive network. Since HSPCs

must be cultivated in serum-free medium to prevent uncontrolled differentiation, tri-cultures were performed

in which MSCs, HSPCs and HUVECs were cultivated in serum-free medium for 1 week. It could be shown that

HUVECs survive in the serum-free medium and maintain primitive vascular structures. Moreover, it is planned

to connect this tissue engineered vascularized dynamic 3D model with the endothelialized channel system of

the MOC, to set up a closed

in vitro

system of a vascularized bone marrow model. This will give the opportunity

for basic research als well as for diagnostics in regenerative medicine more efficiently without animal testing.

Kübrah Keskin et al., J Genet Mol Biol 2019, Volume 3

Kübrah Keskin is a Graduate of Biotechnology and is currently doing her PhD in the Department of Medical Biotechnol-

ogy at the Technical University Berlin.

BIOGRAPHY