world-biochem-regenerative-medicine-2019-scientifictracks

Note:

World Biochem 2019 & Regenerative Medicine 2019

Journal of Genetics and Molecular Biology | Volume 3

Page 44

OF EXCELLENCE

IN INTERNATIONAL

MEETINGS

YEARS

March 25-26, 2019 | Amsterdam, Netherlands

BIOCHEMISTRY AND ENZYMOLOGY

World Congress on

TISSUE ENGINEERING AND REGENERATIVE MEDICINE, STEM CELL RESEARCH

Global Conference on

Joint Event on

MORPHOMETRIC AND HISTOLOGICAL EFFECTS OF AQUEOUS EXTRACT OF

COSTUS AFER STEM JUICE ON CUTANEOUSWOUND OF ADULT ALBINO RATS

Kudighe Patrick Udoh

University of Uyo Teaching Hospital, Nigeria

kin regeneration is a major challenge of adult mammals that has only been overcome with elaborate and ex-

pensive procedures. This calls for the search for cheaper and readily available wound regeneration products.

Thus, this study was to investigate the wound healing potential of costus afer stem juice which has been shown

to contain pro-healing biomolecules and minerals. The study was carried on twenty-five adult female albino rats

weighing between 243-320g. The rats were divided into 5 groups of group A without treatment; group B, treated

only on the first day with iodine ointment; group C, treated for 7days with iodine ointment; group D, treated only

on the first day with costus afer paste; and group E, treated for 7days with Costus afer paste. They were wounded

on their dorsum under ketamine hydrochloride anaesthesia and were treated according to their groups upon

haemostasis. Rate of contraction and re-epithelialization were evaluated digitally. The tensile strength of healed

skin was measured using a tensiometer. Samples of the healed tissue were collected for H&E and Masson’s tri-

chrome histological assessment. It was found that groups D and E wounds treated with costus afer extract main-

tained an uninfected moist wound environment despite being open to the atmosphere. The rate of re-epithe-

lialization was significantly highest in group D which also had a significantly highest tensile strength of 692.2g

at p<0.05. There was no significant difference in the rate of contraction. H&E and Masson’s trichrome sections

showed randomly organized collagen fibres in groups D and E. There was also indication of growing hair follicles

in group D. in conclusion, aqueous extract of costus afer stem prove to be more effective in wound healing than

iodine. With advanced investigations, it could provide a convenient, common and affordable wound healing

agent that can lead to skin regeneration.

Kudighe Patrick Udohl, J Genet Mol Biol 2019, Volume 3

Kudighe Patrick Udoh is a lecturer with the University of Uyo Teaching Hospital, Nigeria. She obtained her BSc. Anatomy

and MSc from the University of Uyo. She is a passionate educator and researcher with research interest in tissue regen-

eration. She seeks to discover natural remedies that can be used effectively for regeneration of loss tissues. Her present

work discovered a plant product that maintains an open moist wound environment and drives the healing process

towards skin regeneration. She has publications in many journals and she is open for research collaboration.

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