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J Clin Exp Tox 2017 Volume 1 | Issue 2
Toxicology and Pharmacology
November 01-02, 2017 | Toronto, Canada
International Conference on
M
arinenovelnaturalproductshavebeenappliedforcancer
therapies. Enzyme-digested gelatin hydrolysates have
proven to serve as promising sources of potent biologically
active peptides. Potential anti-breast cancer properties of
the extracted Ficin-digested gelatin hydrolysate from Indian
squid (
Uroteuthis duvauceli
) extensively characterized by
cellular and animal models. Gelatin was extracted from squid
skin, hydrolyzed by Ficin, and characterized by standard
physio-chemical methods. Ficin-digested gelatin hydrolysate
was used at various doses of 0-0.1 mg/mL for treatments of
MCF-7 and MDA-MB-231 breast cancer cells versus HUVEC
normal cells. Cytotoxicity, phase-contrast morphological
examination, apoptosis/necrosis, clonal-growth, cell-
migration, Matrix-metalloproteinases (MMPs) zymography,
and Western blotting were used for cellular assessments. For
animal studies, breast tumor-induced BALB/c mice received
hydrolyzed gelatin regimen, followed by tumor size/growth
and immune-histochemical analyses. Significant inhibition
of MCF-7 and MDA-MB-231 with no cytotoxicity on HUVEC
cells was detected. Apoptosis was increased in cancer cells,
as revealed by elevated ratio of cleaved caspase-3 and
PARP. MMP-2 and MMP-9 activities in both cancer cells
were dramatically diminished. In mice, gelatin hydrolysate
prevented weight loss, decreased tumor size, induced p53,
and down-regulated Ki67 levels. These findings suggest
that Ficin-digested gelatin hydrolysate could be a beneficial
candidate for novel breast cancer therapies.
Speaker Biography
M Reza Khorramizadeh, is a Full Professor at Tehran University of Medical Sciences
(TUMS), directs Biosensor Research Center and newly instituted Zebra fish Core Lab at
Endocrinology and Metabolic Molecular-Cellular Sciences Institute. Concurrently, he is
a 2
nd
affiliation to the Dept. of Medical Biotechnology, School of Advanced Technology
in Medicine, TUMS.
e:
khoramza@tums.ac.irM Reza Khorramizadeh
Tehran University of Medical Sciences, Iran
Assessment of inhibitory effects of Ficin-hydrolyzed gelatin derived from squid
(
Uroteuthis duvauceli
) on breast cancer cell lines and animal model