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Structural Biology 2018 & STD AIDS 2018
Journal of Genetics and Molecular Biology
|
Volume 2
S e p t e m b e r 0 3 - 0 4 , 2 0 1 8 | B a n g k o k , T h a i l a n d
allied
academies
STD-AIDS AND INFECTIOUS DISEASES
STRUCTURAL BIOLOGY AND PROTEOMICS
&
International Conference on
International Conference on
Joint Event on
Panat Anuracpreeda, J Genet Mol Biol 2018, Volume 2
IMMUNODIAGNOSTIC TEST KITS FOR
RAPID DETECTION OF FASCIOLOSIS AND
PARAMPHISTOMOSIS
Panat Anuracpreeda
Mahidol University, Thailand
Statement of the Problem:
Tropical fasciolosis caused by
Fasciola gigantica
infection and paramphistomosis caused by paramohistomes are the major
diseases infecting ruminants and humans in the tropical regions of Africa
and Asia including Thailand. Parasitological diagnosis of fasciolosis is
often unreliable and possesses low sensitivity. Therefore, the detection of
circulating parasite antigens is thought to be a better alternative for diagnosis
of fasciolosis, as it reflects the real parasite burden.
Methodology & Theoretical Orientation:
In this study, we have produced a
monoclonal antibody (moAb) against native and recombinant antigens and
developed both sandwich enzyme-linked immunosorbent assay (sandwich
ELISA) and immunochromatographic (IC) test for rapid detection of circulating
antigens in the sera or stool from mice experimentally and cattle naturally
infected with
Fasciola gigantica
or paramphistomes.
Findings:
The lower detection limits of sandwich ELISA and IC test were 3 pg/
ml and 0.256 ng/ml, respectively. Sandwich ELISA and IC test could detect
F.
gigantica
infection from day 1 to 35 post infection. In experimental mice, the
sensitivity, specificity and accuracy were 95%, 100% and 98.6% (for sandwich
ELISA), and 93%, 100% and 98.2% (for IC test), while in natural cattle they
were 98.3%, 100% and 99.5% (for sandwich ELISA) and 96.7%, 100% and
99.1% (for IC test).
Conclusion&Significance:
Thesetwoassaymethodsshowedhighefficiencies
and precisions for diagnosis of fasciolosis and paramphistomosis.
Figure.1: An immunochromatographic (IC) strip test is developed for
diagnosis of fasciolosis by
F. gigantica
: experiment trial. (A) A schematic
diagram of the immunochromatographic (IC) strip test showing several
components: a sample pad, a conjugate pad, an
immobilized nitrocellulose membrane (control
and test antibody) and an absorbent pad. (B) The
samples of the IC strip test for deciding the results:
a positive result shows two red dots at the test and
control regions, while a negative result exhibits
only one red dot in the control region. The strip
tests are invalid when there is no red dot at the
control region.
Recent Publications
1. Anuracpreeda P, Kullanid Tepsupornkul,
Chawengkirttikul R (2017). Immunodiagnosis
of paramphistomosis using monoclonal
antibody-based sandwich ELISA for detection
of
Paramphistomum gracile
circulating 16 kDa
antigen. Parasitology. 144: 899-903.
2. Anuracpreeda P, Amaya Watthanadirek,
Chawengkirttikul R, Sobhon P (2017).
Production and characterization of a
monoclonal antibody specific to 16 kDa
antigen of
Paramphistomum gracile
. Parasitol
Res. 116: 167–175.
3. Anuracpreeda P, Chawengkirttikul R, Sobhon
P (2016). Immunodiagnostic monoclonal
antibody-based sandwich ELISA of fasciolosis
by detection of
Fasciola gigantica
circulating
fatty acid binding protein. Parasitology. 143:
1369-1381.
4. Anuracpreeda P, Chawengkirttikul R, Sobhon
P (2016) Antigenic profile, isolation and
characterization of whole body extract of
Paramphistomum gracile
. Parasite Immunol.
38: 431-438.
Panat Anuracpreeda is an Associate Professor of
Mahidol University and belongs to Molecular Medical
Biosciences Cluster. He is associated with Institute
of Molecular Biosciences. He has his research inter-
ests in parasite immuno-molecular biology, advance
hybridoma technology, advance immuno-molecular
diagnostic assays and advance immuno-molecular
therapy.
panat.anu@mahidol.ac.thBIOGRAPHY