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Virology research J 2017 Vol 1 Issue 2
Notes:
July 26-27, 2017 | Vancouver, Canada
WORLD CONFERENCE ON STDs, STIs & HIV/AIDS
allied
academies
Background:
Diagnostic tests are needed for the control of
sexually transmitted infections. Rapid advances in molecular
biology have led to the development of sensitive and
specific diagnostic tests. Immunochromatographic test for
dual diagnosis of both HIV and syphilis by detecting IgG/
IgM antibodies to HIV and recombinant Treponema pallidum
antigens to syphilis testing has recently been developed as a
novel point-of-care testing technology. New diagnostics tests
must be evaluated in the situations in which they will be
deployed. However, this product has yet not been evaluated
in pregnant mothers in Ethiopia.
Method:
A total of 1,888 characterized repository plasma
samples, previously collected from pregnant mothers were
used. HIV ELISA Ag-Ab test (Vironostica) as a screening and
Bio- Meurex as a confirmatory were used as gold standards
for evaluation of HIV, ELISA (Trepanostica-TPPA) recombinant
were also used as a gold standard for syphilis testing. The
samples were assayed for the presence of antibodies to HIV
and T. Pallidum by the SD Bioline HIV/syphilis Duo. Moreover,
the samples were characterized using SPINREACT RPR kit for
syphilis.
Result:
The sensitivity and specificity of SD Bioline HIV/
syphilis Duo in HIV testing were 97.6% and 100%. The
sensitivity and specificity of SD Bioline HIV/syphilis Duo in
detecting syphilis were 95.2% and 97%. Moreover, the kappa
value for SD Bioline HIV/syphilis Duo had
0.99 and 0.616 in HIV and syphilis testing respectively. Inter
reader variability of SD Bioline HIV/syphilis Duo was greater
than 3%.
Concussion
: SD Bioline HIV/syphilis Duo was found to be
highly specific but with limited sensitivity in detecting
both HIV and syphilis. It can also be performed in clinics
without laboratory training and require no equipment or
refrigeration. SD Bioline HIV/syphilis Duo compared favorably
with TPPA and ELISA. SD Bioline HIV/syphilis Duo can be used
for simultaneous screening of HIV and syphilis at antenatal
care settings in Ethiopia.
Key words:
Sensitivity, Specificity, Positive Predictive Value,
Negative Predictive Value
Feasibility and Performance of SD BIOLINE Dual HIV/Syphilis Point-of-Care Test Based Screening
Strategy in Ethiopia
Yimam Getaneh
1
, TesfayeTilahun
2
, Seble Girma
3
, Mulu Gima
3
and
Yemane Abreha
3
Ethiopian Public Health Institute, Ethiopia