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July 18-19, 2019 | Valencia, Spain
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
Asian Journal of Biomedical and Pharmaceutical Sciences
ISSN: 2249-622X | Volume 9
Pharma Summit 2019
2
nd
GLOBAL PHARMA SUMMIT
STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMI-
NATION OF ALPRAZOLAM, CLIDINIUM BROMIDE AND DICYCLOMINE HYDROCHLORIDE
BY RP-HPLC
Lal Prasanth M L
DMWIMS College of Pharmacy, India
A
simple, accurate, precise method was developed for the simultaneous estimation of the Dicyclomine hy-
drochloride, Clidinium bromide and Alprazolam in Tablet dosage form. Chromatogram was run through
Kromasil 250 x 4.6 mm, 5m. Mobile phase containing buffer and Acetonitrie in the ratio of 40:60 was pumped
through column at a flow rate of 1ml/min. Buffer used in this method was OPA buffer at pH 2.8. Tempera-
ture was maintained at 30°C. Optimized wavelength for Dicyclomine hydrochloride, Clidinium bromide and
Alprazolam was 225nm. Retention time of Dicyclomine hydrochloride, Clidinium bromide and Alprazolam
were found to be 2.473min, 3.842min and 5.218min. %RSD of system precision for Dicyclomine hydrochloride,
Clidinium bromide and Alprazolam were and found to be 0.3, 0.7 and 1.2 respectively. %RSD of method preci-
sion for Dicyclomine hydrochloride, Clidinium bromide and Alprazolam were and found to be 0.9, 0.5 and 0.5
respectively. Percent recovery was obtained as 99.86%, 100.33% and 100.45% for Dicyclomine hydrochloride,
Clidinium bromide and Alprazolam respectively. LOD, LOQ values are obtained from regression equations of
Dicyclomine hydrochloride, Clidinium bromide and Alprazolam were 0.41ppm, 0.03ppm, 0.005ppm, 1.24ppm
and 0.10ppm, 0.01ppm respectively. Regression equation of Dicyclomine hydrochloride was y= 8451x+13349,
Clidinium bromide was y= 13323x+6412 and of Alprazolam was y= 90717x + 3843.
Lal Prasanth M L, Asian J Biomed Pharmaceut Sci 2019, Volume 9
Lal Prasanth M L is from DM WIMS College of Pharmacy, India. His research findings mainly focus on stability indicating method
development and validation for the determination of Alprazolam, Clidinium bromide and Dicyclomine hydrochloride by RP-HPLC.
lalprasanth@yahoo.comBIOGRAPHY