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Journal of Clinical Pathology and Laboratory Medicine | Volume 3
July 05-06, 2019 | Paris, France
Pathology and Surgical Pathology
2
nd
International Conference on
Notes:
F
ascioloidosis is a parasitic disease caused by non-native
parasite
Fascioloides magna. F. magna
originally parasitize
in the North American deer species. It was introduced to
Europe at least twice, forming the three foci of infection:
Italian, Czech and Danubian. The latter two gave origin to
other, newly formed areas, including the Croatian one.
F.
magna
was detected in Croatia in 2001 and since then have
spread to the majority of its lowland areas. The fact that
F. magna
is a non-native parasite enables us to observe
development of host-parasite interactions, but also to create
conditions of case-control study in the wild, large mammal
population. In this research we aimed to compare variability
and presence of specific alleles of MHC class II – DRB exon
2 in relation to
F. magna
infection. A total of 117 red deer
livers and faecal samples originating from lowland (Baranja,
Lipovljani and Spačva) and mountain area (Gorski Kotar) were
collected. Of them, based on location and parasitological
analysis, 46 individuals were selected and tissue samples were
sent for analysis using next generation sequencing (NGS). We
have detected 44 DRB alleles (2-4 alleles per individual). Four
alleles were already known (our references - DRB_NS36, DRB_
NS38, DRBref01 i DRBref02). Statistically significant difference
was detected in the level of infection in the case of animals
with DRB_ref01 and DRB_ref06 alleles (χ2 = 0, 939, p = 0, 332).
This implicates on a potential development of host-parasite
interaction in the case of red deer and
Fascioloides magna
.
Speaker Biography
Dean Konjevic is currently working as a professor at the department
of veterinary economics and epidemiology, University of Zagreb
Veterinary Faculty. He received his DVM in 2000 and PhD in 2009. He
is active diplomate of the ECZM and European veterinary specialist in
zoological medicine. Since 2016, he serves as vice-dean for science,
postgraduate studies and lifelong learning. He was member of scientific
and organizational committees of several international conferences,
workshops and summer schools. He was principal investigator on
two Croatian Science Foundation projects and one ESF project and
associate on FP7 and IPA projects. His fields of interests are veterinary
epidemiology, wildlife diseases, species conservation and comparative
odontology.
e:
dean.konjevic@vef.hrDean Konjevic
1
Miljenko Bujanic
1
, Franjo Martinkovic
1
, Haidi Arbanasic
1
,
Elena Bužan
2
and
Ana Galov
1
1
University of Zagreb, Croatia
2
University of Primorska, Slovenia
Red deer fascioloidosis – a model for host-parasite interaction