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Journal of Pathology and Disease Biology | Volume 2
September 06-07, 2018 | Edinburgh, Scotland
Pathology and Surgical Pathology
International Conference on
Anti-Cancer drug NSC‐631570 (Ukrain): Induction of the apoptosis in cancer cells
Wassil Nowicky
Ukrainian Anti-Cancer Institute, Austria
In the study on the erythroleucemia cells K‐562, it was
revealed NSC‐631570 to bring about the bimodal death
of cancer cells. At lower concentrations of NSC‐631570,
cancer cells die in as a consequence of apoptosis. At higher
concentrations, the formation of microtubules is inhibited
and polyploidy occurs.
In the tests on human cervix carcinoma cells HeLa, squamous
carcinoma cells WHCO5, normal kidney cell line Graham 293,
and transformed kidney cell line Vero from African green
monkey the researchers of the University of Pretoria, South
Africa revealed NSC‐631570 ‘is selectively toxic to malignant
cells by causing a metaphase block which is characterised
by abnormal chromosomal distribution, and results in the
formation of micronuclei and in apoptosis.
The scientists of the Eberhard Karl University (Tübingen,
Germany) investigated the effect of NSC‐631570 on the
cell survival, the cell cycle modification and the apoptosis
induction alone and combined with radiation (IR). They
discovered NSC‐631570 combined with IR increased the
toxicity against the cell lines CCL‐221 and U‐138MG. The
normal human skin and lung fibroblasts were protected from
the damaging effects of IR.
Estimating the cell proliferation according to the BrdU
uptake in the cell lines AsPC1, BxPC3, MiaPaCa2, Jurkat, and
THP‐1 and the cell cycle phases by means of Giemsa staining,
the authors established NSC‐631570 at a dose of 10 μg/ml
brought about a considerable accumulation of cancer cells
in the G2/M phase after 24 h incubation. The apoptosis rate
in the peripheral mononuclears was similar at the same
incubation conditions. Moreover, the mitogene stimulated
lymphocytes showed increased blastogen reaction.
Effect of NSC‐631570 on cell survival and apoptosis in the
androgen‐independent prostate cancer cell line PC‐3 was
studied. Cell viability was assessed using the dimethyl
thiazolyl tetrazolium bromide (MTT) method in PC‐3 cells after
treatment with Ukrain. The IC50 value was observed in 10 μg
concentration of Ukrain. Bax, Bad, and FasL mRNA expression
was analyzed by reverse transcriptase‐polymerase chain
reaction, and protein expressions of p‐Akt, Bcl‐2, and caspase
10 were determined by western‐blot analysis. Nuclei were
stained with 4’,6‐diamidino‐2‐ phenylindole, dihydrochloride
(DAPI). NSC‐631570 significantly increased the pro‐apoptotic
mRNA expression of Bad, Bax, and FasL; decreased the cell
survival protein p‐Akt and the anti‐apoptotic protein Bcl‐2;
and increased the protein levels of cleaved poly(ADP)‐ribose
polymerase (PARP) and caspase‐10. The results of this study
suggest that NSC‐631570 decreases the cell survival of
androgen‐independent prostate cancer cells.
Speaker Biography
Wassil Nowicky, Director of “Nowicky Pharma” and President of the Ukrainian Anti-
Cancer Institute (Vienna, Austria). Has finished his study at the Radiotechnical faculty
of the technical University of Lviv (Ukraine) with the end of 1955 with graduation to
“Diplomingeniueur” in 1960 which title was nostrificated in Austria in 1975. Inventor of
the anticancer preparation on basis of celandine alkaloids “NSC-631570”. Author of over
300 scientific articles dedicated to cancer research. Dr. Wassil Nowicky is a real member
of the New York Academy of Sciences, member of the European Union for Applied
Immunology and of the American Association for scientific progress, honorary doctor of
the Janka Kupala University in Hrodno, doctor “honoris causa” of the Open international
university on complex medicine in Colombo, honorary member of the Austrian Society
of a name od Albert Schweizer. He has received the award for merits of National guild
of pharmacists of America. the award of Austrian Society of sanitary, hygiene and public
health services and others.
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