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February 28-March 01, 2019 | Paris, France
Palliative Care, Obstetrics and Gynecology
Stroke and Clinical Trials
International Conference on
Joint Event on
International Conference on
&
Journal of Research and Reports in Gynecology and Obstetrics | Volume: 3
Altered gene expression of remodelling proteins after IVF/ICSI explain increased pregnancy
complications
Özlem Gün Eryilmaz
Zekai Tahir Burak Women’s Health and Research Hospital, Turkey
Introduction:
In IVF/ICSI pregnancies, placental adaptation
that favors fatal development may be disrupted due to the
unnatural pathways induced by in vitro interventions. This
may be reason why the incidence of placental complications
such as abnormally adhesive placenta and preeclampsia/
placental ablation are increased in these patients.
Matrix metalloproteinases (MMPs) are proteolytic enzymes
that break up extracellular matrix (ECM) to remodel the
tissues via angiogenic and apoptotic pathways. So called the
tissue inhibitors of metalloproteinases (TIMPs) inhibit MMP
actions and both of them are in balance during healthy tissue
functions. Disturbed balance between MMP and TIMPs in
favor of increased degradative activity during implantation,
placentation and remodelling of the growing placenta may
cause gestational problems which are more common in
the pregnancies conceived via IVF/ICSI. The ADAMTS (A
Disintegrin and Metalloproteinase with Thrombospondin
motifs) enzymes are similar extracellular enzymes likeMMPs.
They cleave or modify the ECM, similar to their relatives.
Increased expression is associated with uncontrolled tissue
proliferation and behaviours of metastatic invasion in
cancer and inflammatory diseases. ADAMTS 1, being the
first identified member of this family, has an additional anti-
angiogenic effect.
The aim of this study was to measure gene expression levels
of MMP2, MMP9, TIMP 1 and ADAMTS 1 in placentas of IVF/
ICSI conceptions and compare them to the gene expression
levels of placentas obtained from control patients who had
conceived spontaneously.
Material Methods:
A total of eighteen tissue samples
of placenta from IVF/ICSI (n=9) and spontaneous (n=9)
pregnancies were obtained. We measured the gene
expression levels of ADAMTS1, MMP2, MMP9 and TIMP1
by real-time polymerase chain reaction. Expression levels
were analysed using the delta threshold cycle method.
Results:
The levels of ADAMTS1, MMP2, MMP9 were
increased in placentas obtained from IVF/ICSI pregnancies
compared to controls (p<0.05 for all). TIMP1 values were not
different.
Conclusions:
Gene expression of MMP2, MMP9 and ADAMTS
1 were increased in the placentas of the pregnancies
conceived via IVF/ICSI compared to those conceived
spontaneously. Disturbed placental architecture as a result
of the increased destructive actions of MMP and ADAMTS
may be one of the reasons why IVF/ICSI pregnancies are
more prone to gestational complications.
Speaker Biography
Özlem Gün Eryilmaz has completed her PhD at the age of 24 years from Hacettepe
University and Ob&Gyn Education and Research Hospital, Turkey. She has been
working as a gynecologist for 22 years and for the last 8 years she has been interested
in IVF and endometriosis. Her publications are mostly over these topics. Since 2013,
she has been working as an associate professor in a government hospital in Ankara.
e:
drozlemgun@gmail.comÖzlem Gün Eryilmaz
, Res Rep Gynaecol Obstet, Volume 3
DOI: 10.4066/2591-7366-C1-003