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N o v e m b e r 2 1 - 2 2 , 2 0 1 8 | M a d r i d , S p a i n
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alliedacademies.comYEARS
Nephrology 2018
Journal of Clinical Nephrology and Therapeutics
|
Volume 2
NEPHROLOGY AND UROLOGY
International Conference on
J Clin Nephrol Ther 2018, Volume 2
SUBCLINICAL EHRLICHIOSIS: MAY CAUSE KIDNEY ALTERATIONS?
Leandro Z Crivellenti
Universidade de Franca, Brazil
W
e hypothesized that renal glomerulopathy is a common finding in dogs with subclinical ehrlichiosis and pro-inflammatory
cytokines may be associated to renal injury. The aim of this study was to evaluate renal cortex biopsies in 15 dogs with sub-
clinical ehrlichiosis diagnosed by PCR and enzyme-linked immunosorbent assay (ELISA) and in 17 healthy dogs as a control group.
Dogs with presence of clinical signs, comorbidities and/or azotemia were excluded. Biopsy material was examined by light micros-
copy (LM). Sections were stained with hematoxylin and eosin, periodic acid Schiff, Jones methenamine silver, Masson´s trichrome,
and Congo Red. Cytokine quantification of tumor necrosis factor alpha (TNF-α), interferon gamma (INF-γ) and interleukin 6 were
assessed through ELISA using commercial kits specific to dogs (Milliplex), according to the manufacturer’s instructions. LM abnor-
malities were identified in 14 dogs (93.3%) from the ehrlichiosis group, but most findings were subtle. Mesangial cell proliferation
(40.0%), synechiae (40.0%), globally sclerotic glomeruli (33.3%), ischemic glomeruli (33.3%), focal segmental glomerulosclerosis
(FSGS) (33.3%), focal thickening of the glomerular basement membranes (26.7%), hydropic degeneration (26.7%) and interstitial
fibrosis and tubular atrophy (IFTA) (20.0%) were frequent. Cytokines was increased in subclinical phase on levels of TNF-α, INF- γ,
and IL-6 in comparison to control. These results suggest that TNF-α, INF- γ and -IL-6 may be involved in the pathogenesis of tu-
bule-glomeruli injury and subclinical ehrlichiosis may lead in chronic kidney disease (acknowledgement to FAPESP for financial
support - #2014/21506-2).