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Journal of Materials Science and Nanotechnology | Volume 3
May 16-17, 2019 | Prague, Czech Republic
2
nd
International Conference on
22
nd
International Conference on
Nanomaterials and Nanotechnology
Advanced Nanoscience and Nanotechnology
Joint Event
&
Mater Sci Nanotechnol, Volume 3
B
iosensors have drawn much attention because of their
great potential to facilitate biomedical research, drug
discovery, environmental monitoring and diagnosis of
diseases. A powerful bio-detection requires highly specific
bio-recognition probes and sensitive elements. Several
techniques, such as gold nano-particles (AuNPs), quartz
crystal microbalance (QCM) and surface plasmon resonance
(SPR), silicon nanowire field-effect transistor (SiNW-FET)
and others have been extensively studied to improve the
sensitivity of biosensing. Antibody is commonly used to
conjugate with a sensing chip for a large molecule detection.
Yet, for small molecule detection, new strategies need to be
developed. We’ll demonstrate a conventional method, based
on protein engineering, to produce a bio-recognition probe
and to construct an effective device for quantitatively sensing
steroids. We’ll further exhibit an open-sandwich immuno-
recognition system containing VH and VL of a scFv for small
molecules detection. The powerful technique for screening
scFv from phage display library will also be discussed.
Speaker Biography
Yaw-Kuen Li received his PhD degree from Tulane University, USA, in 1991.
After his postdoctoral research in School of Medicine of Johns Hopkins
University, he moved back to Taiwan to start his academic career in 1993.
He was promoted to the full professor in 2002. Further, he became the
chair of the department (2004-2006) and the dean of college of science of
National Chiao Tung University (2014-2017). His primary research interests
include three major fields: Enzyme-based catalytic biological reactions, bio-
recognitionand bio-sensors and solid-state/biological interface chemistry.
e:
nctuykl@g2.nctu.edu.twYaw-Kuen Li
National Chiao Tung University, Taiwan
Biorecognition and biosensing: From big to small