8 / 9
allied
academies
Page 33
Notes:
Journal of Materials Science and Nanotechnology | Volume 3
May 16-17, 2019 | Prague, Czech Republic
2
nd
International Conference on
22
nd
International Conference on
Nanomaterials and Nanotechnology
Advanced Nanoscience and Nanotechnology
Joint Event
&
Mater Sci Nanotechnol, Volume 3
I
ce-bindingproteins(IBPs)areproteinsthatincludeantifreeze
proteins (AFPs) on the one hand and ice-nucleating proteins
(INPs) on the other hand. IBPs are found in organisms
that live under subfreezing temperature conditions. IBPs
depress the freezing point of the body fluids that prevents
freezing of the organism in supercooled conditions, inhibits
ice recrystallization, enable adhesion to ice and promote
nucleation depending on their size. We are investigating
the interactions of IBPs with ice surfaces. For example, we
study the dynamic nature of the protein/ice interaction
using fluorescence microscopy techniques combined with
temperature-controlled microfluidic devices. The results
show that binding of IBP to ice is irreversible, that the freezing
temperature depression is sensitive to the time allowed for
the proteins to accumulate on ice surfaces, and the distance
between the proteins to be down to few nanometres. Our
studies also revealed that IBPs can function in temperatures
as low as -100
o
C, thus suitable for cryopreservation. We also
found that the small IBPs adhere to ice and inhibit its growth,
while the big IBPs nucleate new ice crystals. These results
contribute to an understanding of the mechanisms by which
the nanometric IBPs control ice growth and are critical for the
successful use of IBP in cryobiological applications.
Speaker Biography
Ido Braslavsky is currently working as an associate professor at The
Hebrew University of Jerusalem, Israel. He is also the head of BSc Program
in Biochemistry and Food Science, Institute of Biochemistry, Food Science,
and Nutrition. He is also the member of the Governors of the Society of
Cryobiology. His research mainly focuses on ice growth and its control. His
PhD studies were on Ice Physics at the Israel Institute of Technology. After
postdoc positions at the Weizmann Institute of Science and at Caltech,
where he conducted biophysics studies on DNA –proteins interactions and
developed single-molecule DNA sequencing method, he initiated a study
on ice-binding proteins biophysics at Ohio University Physics department.
In the last ten years, his group published more than 30 papers on ice-
binding proteins and ice growth control. His research has been supported
by the National-Science-Foundation (NSF) and the Israel-Science-
Foundation (ISF) and the European-Research-Council (ERC).
e:
ido.braslavsky@mail.huji.ac.ilIdo Braslavsky
The Hebrew University of Jerusalem, Israel
Proteins that bind ice and their control on freezing