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Microbiology: Current Research | Volume 3
May 20-21, 2019 | Vienna, Austria
Medical Microbiology
4th International Conference on
The use of naturally occurring phages in agriculture and
medicine has flourished for years and now, is continuing
to enable novel strategies in synthetic biology. Synthetic
biology has refined the way to design modify and enrich to
optimize by rationally engineering the phages. New function
can be achieved by fusing libraries comprising synthetic
peptides with a coat protein of a phage and such peptide is
displayed on the phage surface. Phage engineering was made
possible with the advances of DNA recombinant technology
and was mostly applied to DNA phages. RNA phages possess
features that can accelerated evolution and serve as platform
or tool phage-based for in vitro evolution. RNA phages RNA-
dependent-RNA-polymerase enzyme lacks the proof reading
activity and this contributes to the genotypic and phenotypic
divergence, convergence of evolution and could improve the
optimization of any engineering efforts. RNA-Coliphage Qβ
has been, is and will be great interest and fascinating platform
for evolutionary synthetic biology. Qβ is also an important tool
to map the library. Recently, we have successfully constructed
and exposed a 5-mer-library of FMDV VP1 G-H loop on the
surface of Qβ. The tandem amino acid motif that is required
for anti-FMDVmonoclonal antibodywas selected and evolved
with our novel panning system. Epitopes of SARS-CoV spike-
proteins were mapped, using Qβ and evaluated for potential
antibody neutralizing determinants critically important in
the development of an efficacious vaccine candidate. We
have demonstrated that recombinant Qβ framed with gp41
MPER of HIV can be used either alone or in combination
with other strategies for the production and monitoring
of HIV-1 gp41 MPER-specific immune responses. With the
volume of reports and papers on RNA viruses’ replication
to antiviral therapy and escaping immune molecules, the
struggle and difficulties to develop vaccines against these
viruses can be alleviated using RNA phage display system.
Speaker Biography
Alain B Waffo has completed his PhD at the age of 33 years from Max-
Planck-Institute of Biophysical Chemistry of Goettingen, Germany under
the supervision of Profs. Manfred Eigen and Biebricher Christof. He has
two postdoctoral trainings in Newark in New Jersey Medical School and
in Texas Medical Center. He is the director, advisor of the Biomedical and
Capstone programs and associate professor of Alabama State University,
USA. His work has been devoted on infectious diseases caused by RNA
virus. His research is sponsor by DoD, NIH and NSF. He has over 50
publications that have been cited over 400 times, and his publication
H-index is 17 and has been serving as reviewer and an editorial board
member of reputed Journals.
e:
Alain B Waffo
Alabama State University, USA
Novelties in phage display with RNA-coliphage Qβ for diseases
point-of-care
Alain B Waffo, Microbiol Curr Res, Volume 3
DOI: 10.4066/2591-8036-C1-004