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Materials-Metals 2017
Page 33
November 16-17, 2017 Paris, France
13th Annual Conference on
Materials Science, Metal and Manufacturing
Journal of Materials Science and Nanotechnology
Volume 1 Issue 2
Kaoru Tamada et al., Mater Sci Nanotechnol 2017, 1:2
High-resolution imaging of a cell-attached
nanointerface using a gold-nanoparticle two-
dimensional sheet
Kaoru Tamada1, Shihomi Masuda 1, Sou Ryuzaki1, Pangpang Wang1, Koichi
Okamoto1, Thasaneeya Kuboki1, Satoru Kidoaki 1, Yuhki Yanase2 and Eiji
Usukura3
1Kyushu University, Japan
2Hiroshima University, Japan
3Nagoya University, Japan
This paper reports our original technique for visualizing
cell-attached nanointerfaces with extremely high axial
resolution (a few tens of nanometers) using collectively excited
localized surface plasmon resonance (LSPR) on a self-assembled
metal nanoparticle sheet. Oleylamine-capped gold NPs (AuOA,
13nm) and myristate-capped silver nanoparticles (AgMy, 5nm)
were self-assembled at an air-water interface and transferred on
hydrophobic cover slip by Langmuir-Schaefer method to be an
imaging substrate. This self-assembled metal nanoparticle sheet
can confine and enhance the fluorescence at the nanointerface.
Test experiments on rat basophilic leukemia (RBL-2H3) cells
with fluorescence-labeled actin filaments revealed high axial and
lateral resolution in the image of focal adhesionat the cell-attached
interface even under a regular epifluorescence microscope,
which produced higher quality images than those captured
under a total internal reflection fluorescence (TIRF) microscope.
Recently, the demand for super-resolution fluorescence
microscopy is increasing in the field of cell biology because of the
requirement to investigate molecular-level dynamic reactions in
or near cells. The super-resolution microscopy techniques, such
as confocal laser microscopy, STED, SIM, and PALM/STORM,
have a significant advantage in their lateral resolution but are
not as advantageous in either their axial resolution or temporal
resolution because of their scanning criteria. TIRF microscopy
provides the highest axial and temporal resolution compared
with the other super-resolution microscope systems, although
the imaging area is still 100-200 nm from the top surface of
a cover slip. The common problem of these state-of-the-art
technology is the cost of the apparatus, which prevents it from
being standard equipment in basic laboratories. On contrast,
our non-scanning-type, high-resolution imaging method using
nanoparticle LSPR is very user-friendly and effective tool for
monitoring nano interfacial phenomena. This technique will
open the possibility for all biochemists and medical scientists
to perform state-of-the-art molecular imaging using their own
conventional microscope.
Figure: (a) Schematic drawing of the depth of the LSPR on the
AuOA sheet and evanescent fields on glass. (b) Fluorescence
images of FITC-labeled actin filaments in RBL-2H3 cells on the
AuOA sheet (left) and on glass(right).
Recent Publications
• S Masuda et. al. (2017) High-resolution imaging of a cell-
attached nanointerface using a gold-nanoparticle two-
dimensional sheet. Sci. Rep. 7:3720.
• M Toma et. al. (2011) Collective plasmon modes excited
on a silver nanoparticle 2D crystalline sheet. Phys. Chem.
Chem. Phys. 13(16):7459-7466.
• D Tanaka et. al. (2015) Characteristics of localized surface
plasmons excited on mixed monolayers composed of self-
assembled Ag and Au nanoparticles.
• K Okamoto et. al. (2016) Electromagnetically induced
transparency of a plasmonic metamaterial light absorber
based on multilayered metallic nanoparticle sheets. Sci. Rep.
6:36165
Biography
Kaoru Tamada is a Scientist in the field of Surface Science and Nanoscience.
After 7 years of R&D experience in industry, she joined Prof. Hyuk Yu’s lab in
Univ. of Wisconsin-Madison and obtained Dr. Sci. at Nara Women’s University
in 1994. After Postdoc experience in Riken, she worked as a Senior Scientist in
AIST Japan for 10 years. During this period, she joined ANU, MPIP and NUS as
a Visiting Scientist. She joined TokyoTECH in 2005 as an Associate Professor,
and RIEC, Tohoku Univ. in 2007 as a Professor. She moved to IMCE, Kyushu
Univ. in 2011, and was promoted to Vice President from 2017. Her research
interest is self-assembly of molecules and nanomaterials, plasmonics, and their
bio-sensor and bio-imaging applications.