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Journal of Materials Science and Nanotechnology | Volume: 2
August 20-21, 2018 | Paris, France
Materials Science and Materials Chemistry
International Conference on
Degradation kinetics and stability indicating RP-HPLC method for the estimation of flavoxate
hydrochloride in bulk and pharmaceutical dosage forms
K Srinivasa Rao
Indira Gandhi National Tribal University, India
A
simple, rapid, economic stability indicating reversed-phase
HPLC method was developed, validated and subsequently
degradation kinetics in acidic, alkaline, oxidative, thermal,
photolytic media are assessed for Flavoxate Hydrochloride
(FVH) present in pharmaceutical dosage forms. The proposed
RP-HPLC method utilizes a LiChroCART-Lichrosphere100, C18
RP column Hibar (250 x 4 mm, 5μm) in an isocratic separation
mode with mobile phase consisting of methanol and water in
the proportion of 50:50 % (v/v), at a flow rate of 0.8 ml/min
and the effluent was monitored at 315 nm. The retention
time of FVH was found to be 2.92 min. Stability of FVH was
investigated as per ICH-prescribed stress conditions. Significant
degradation of FVH was observed under all studied stress
conditions. A kinetic study was conducted to investigate
the degradation kinetics of FVH at different temperatures;
reaction rate constants, half-life times and activation energy
were calculated in all the media. The described method was
linear over a range of 1-300µg/ml. The percentage recovery
was 99.46. F-test and t-test at 95% confidence level were
used to check the intermediate precision data obtained under
different experimental setups; the calculated value was found
to less than the critical value. The proposed method can thus
be used for routine analysis, quality control and for studies of
the stability of pharmaceutical tablets containing these drugs.
e:
ksrao108@gmail.com