material-science-germany-2019-scientifictracks

9 / 13

allied
academies
Page 31
Notes:
Journal of Materials Science and Nanotechnology | Volume 3
October 07-08, 2019 | Frankfurt, Germany
Materials Science and Engineering
3rd International Conference on
Mater Sci Nanotechnol, Volume 3
2-Hydroxyethyl Methacrylate (HEMA) treatment modulates the autophagic process in
stem cell from human dental pulp trough ERK/pERK signalling
Guya Diletta Marconi and Jacopo Pizzicannella
University D’Annunzio Chieti-Pescara, Italy
Autophagy is an intracellular process that degrades
organelles or cellular components in order to ensure
the maintenance of cell homeostasis. It can be considered
a genetically programmed, adaptive response to stress. In
restorative dentistry practice, free resin monomers of can be
released. The aim of this study was to investigate the effect of
HEMA on proliferation and autophagy in human dental pulp
stemcells (hDPSCs). HumanDPSCswere treatedwithdifferent
concentrations of HEMA (3 and 5 mmol L-1). To evaluate
the proliferation rate, MTT and trypan blue assays were
used. Autophagic markers such as microtubule-associated
protein 1 light chain 3 (LC3-I/II) and ubiquitin-binding
protein (p62) were analyzed through immunofluorescence
observations. Beclin1, LC3-I/II, and p62 were evaluated
by means of Western blotting detection. Considering that
activity of extracellular signal-regulated kinase (ERK) and
its phosphorylated form (pERK) mediates several cellular
processes, such as apoptosis, autophagy, and senescence, the
involvement of ERK/pERK signaling was also evaluated. Our
results showed a decreased cell proliferation associated with
morphological changes in HEMA-treated cells. The Western
blot results showed that the expression levels of Beclin1,
LC3-I/II, and ERK were significantly elevated in HEMA-treated
cells and in cells co-treated with rapamycin, an autophagic
promoter. The expression levels of p62 were significantly
reduced compared to the untreated samples. Protein levels
to the autophagic process, observed at confocal microscopy
confirmed the data obtained from the Western blot. The up-
regulation of ERK and pERK levels, associated with nuclear
translocation, revealed that ERK pathway signaling could act
as a promoter of autophagy in dental pulp stem cells treated
with HEMA. Then in response to HEMA injury, dental pulp
stem cells activate autophagy as a pro-survival cytoprotective
mechanism. Further studies are necessary to consider the
strategic and therapeutic applications of this research in
tissue repair and regeneratio.
Speaker Biography
Guya Diletta Marconi is as Researcher in Biomedical field at Department
of Medical, Oral and Biotechnological Sciences, University of Chieti, Italy.
She spent one year as a postdoctoral researcher at Torrey Pines Institute
for Molecular Studies, in San Diego. She received her Ph.D. in April 2016
in Drug Sciences at University of Chieti, she spent half or her PhD abroad
as visiting PhD student at the Sanford Burnham Prebys Medical Discovery
Institute in San Diego, where she extended her skills and experience with
a focus on the identification and validation of peptides inhibitors of target
proteins involved in cancer progression. She works with multidisciplinary
group that carry highly translational basic science research. The laboratory
of Prof. O. Trubiani, where she works as a researcher, used mesenchymal
oral stem cells, aimed to validate novel bioactive scaffolds as promising
new approaches for tissue regeneration and repair. The current research
is focused on mechanisms involved in stem cell-regulated tissue
homeostasis and repair, particularly on the identification of molecules
and phenotypic changes responsible for the regulation of the stem cell
regenerative potential.
e: