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allied
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MASS SPECTROMETRY
AND PROTEOMICS
International Conference on
J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d
Journal of Systems Biology & Proteome Research
|
Volume 2
Page 15
Note:
S
ynthetic and polymeric oligonucleotides (RNA, DNA and their analogs)
have been developed in recent many years as therapeutic drugs against
a wide range of diseases conditions. During the studies, a challenge step is
bioanalytical analysis. Although Scientists have used many technologies,
for example gels, capillary electrophoresis, high-resolution ion exchange
chromatography and MALDI etc. for the analysis in biofluids since 1970s,
successful bioanalytical technologies have been eventually focused on
ELISA and LC-MS/MS methodologies. However the ELISA assay cannot
distinguish, in the most cases, full length parent oligonucleotides from
shortened species of their metabolites or other endogenous molecules, thus
preventing it from being widely utilized in metabolism studies, especially for
quantitation analysis. In our laboratories, we have developed or validated LC-
MS/MS Methods for 13-mer to 20-mer oligonucleotides (and analogs) which
are more accurately and specifically than ELISA methods developed at our
laboratories in biological matrixes, e.g. plasma, urine, and tumor samples. In
this presentation, our specified extraction procedures of oligonucleotides from
plasma: liquid-liquid, solid phase SPE, and immunoprecipitation extractions
will be discussed. Meanwhile LC-MS/MS conditions, ion pair reagents in UPLC
and multi-charge species situation in mass spectrometer will be presented.
Under our optimized conditions, 10-20 ng/mL of LLOQ were reached which
is the one of most sensitive method. Methods are used for pre-clinical and
clinical sample analysis in our laboratories.
Biography
Yong-Xi Li has completed his Postdoctoral train-
ings at Kansas State University, Cornell University,
USA. Currently, he is Executive Director at Med-
pace Bioanalytical Laboratories after he served
as vice presidents at XenoBiotic Labs and Ricerca
Bioscience. His experiences are focusing on bio-
analysis: TK, PK, ADA, NAB (and Cell base Nab),
PD markers including method developments, val-
idations, sample analysis for small molecule, pro-
tein and antibody therapies. He and his group de-
velopedmany such applications by using LC-MS/
MS and immunoassays (ELISA, ECL and Flow
cytometry......). He is author, co-author of more
than 150 papers, book, presentations in reputed
journals, and conferences. He is also serving as
an organizing committee member for one of bio-
tech conferences.
y.li@medpace.comA SENSITIVE METHOD FOR
QUANTITATIVE ANALYSIS OF
OLIGONUCLEOTIDE THERAPEUTIC
DRUG IN HUMAN PLASMA BY LC-MS/
MS METHODOLOGY
Yong-Xi Li
Medpace Bioanalytical Laboratories, USA
Yong-Xi Li, J Syst Biol Proteome Res 2018, Volume 2