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Infectious Diseases Congress 2019
Journal of Bacteriology and Infectious Diseases | Volume 3
June 12-13, 2019 | Bangkok, Thailand
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
BACTERIOLOGY AND INFECTIOUS DISEASES
2
nd
Global Congress on
J Bacteriol Infec Dis 2019, Volume 3
GENOME CHARACTERISTICS OF A
CAMPYLOBACTER JEJUNI
63A ISOLATED FROM CALI-
FORNIA GULL EXCRETA
Jingrang Lu
1
, Scott P Keely
1
, Sharon Yelton
2
and
Nicholas Ashbolt
1
1
US EPA National Exposure Research Laboratory, USA
2
Dynamac Inc., USA
C
ampylobacter jejuni
is not only a major cause of human gastroenteritis in western countries due to food and
water contamination, but it is also associated some outbreaks of Guillain-Barre´syndrome (GBS).
C. jejuni
63A
was isolated from California gull excreta, which was in the meanwhile found to be a major strain of
Campylobacter
species in Sandhill crane (a migratory bird) excreta as well. The novel isolate is 99% identical to
C. jejuni
strain ICD-
CCJ07001, which was isolated from a GBS patient according to several gene sequences of mapA, aspA, atpA, glnA,
glyA and tkt. The genome of 63A was sequenced with an Illumina HiSeq sequencing platform, assembled and
compared with the strain ICDCCJ07001. It revealed that the 63A genome consisted of 1,697,260 base pairs (bp)
with the GC content 30.47%. The virulence loci and virulence-associated genes evaluated were found to be 98-
99% identical to the equivalent genes in ICDCCJ07001. Some sequence differences were found in 63A compared
to ICDCCJ07001. For example, eight differences were observed in Invasion Antigen B, 22 were observed in cadF,
and 12 were observed in surface-exposed lipoprotein (jlp A). Strain-specific assays of the 63A based on prophage
similar sequences were designed, which could be used for some water fowl
C. jejuni
source tracking and further
pathogenic animal model experiment.