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allied
academies
Allied J Med Res 2017
Volume 1 Issue 2
Herbal Medicine 2017
September 01-02, 2017 London, UK
3
rd
International Conference and Expo on
Herbal & Alternative Medicine
Notes:
Page 33
Evaluation of antioxidant and polyphenolic
content of a Sri Lankan poly herbal formulae
and assessment of its
in vitro
antiproliferative
activity and mechanism of action on RD and
MCF-7 cancer cells compared to healthy CC1
cells
Wageesha N D A
1, 2
, Soysa P
2
, Keerthi, AAP
1
, Choudary, I
3
and
Ekanayake M
4
1
South Asian Institute of Technology & Medicine, Sri Lanka
2
University of Colombo, Sri Lanka
3
University of Karachi, Pakistan
4
No 9, Moragahapitiya, Balagola, Kengalle, Kandy, Sri Lanka
Objectives:
The objective of the current study is to
evaluate the presence of antioxidants, polyphenolics in a
traditional Sri Lankan poly herbal drug and determination
of its anti-proliferative activity.
Materials & Methods:
The total phenolic content (TPC)
and antioxidant activity was determined
in vitro
. The cell
viability was determined by MTT assay. Brine shrimp
assay and LDH leakage was conducted to determine the
cytotoxicity. GSH content were assayed to determine the
oxidative stress exerted by LPG. Rhodamine 123 staining,
caspase 3 activity, DNA fragmentation were used to
identify the apoptosis mechanisms associated with LPG.
RD cells, MCF-7 cells and, CC1 cells were used in all
experiments.
Results:
The TPC% of the LPG was 5.31±0.14% of
GAE and antioxidant capacity is comparable to ascorbic
acid. LPG exhibited strong cytotoxic activity against RD
and MCF-7 cell lines with MTT assay. A 50% leakage of
LDH was observed at concentrations less than 30 μg/mL
and 10 μg/mL for both RD and MCF-7 cells respectively
after 24 hour exposure. While, LPG exhibited strong
cytotoxic activity against RD and MCF-7 cells, the brine
shrimp and CC1 cells results (EC50>100 μg/mL) suggest
that the LPG have minimum cytotoxicity towards the
normal healthy cells. The reduction of GSH content and
elevation of cell survival with exogenous GSH prove that
the LPG act via induction of oxidative stress. Rhodamine
123 assay shows the mitochondrial involvement in cell
death by depletion of Δψ inducing downstream events
in apoptosis only in RD cells. This results in increase in
caspase-3 activity eventually LPG induced apoptotic cell
death. Disparity to RD cells, the MCF-7 cell does not show
any features of apoptosis even in the presence of high
concentrations of LPG.
Conclusion:
In conclusion, the present study suggested
that the LPG exerted an anti proliferative activity via
oxidative stress dependent apoptosis in RD cells but not
in MCF-7 and healthy CC1 cells.
Biography
Wageesha N D A received his Undergraduate Education from the Institute of
Chemistry Ceylon, Sri Lanka, and his MPhil in Biochemistry from University
of Sri Jayewardenepura, Sri Lanka. He is currently a Senior Lecturer in
Biochemistry and Chemistry at Department of Biochemistry and Chemistry,
the Faculty of Medicine, South Asian Institute of Technology and Medicine, Sri
Lanka. His current research interest involves cancer research and is currently
pursuing his PhD in the field of development of novel anti-cancer drug based
on traditional medicinal knowledge. His work has been presented at scientific
conferences and published in journals. He received “Kandiah Graduateship
Award” for Post Graduate research from the Institute of Chemistry, Ceylon in
2010 for his research work.
awageesha@hotmail.comWageesha N D A et al., Allied J Med Res 2017