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Hematology and Blood Disorders | Volume 2
Page 27
July 25-26, 2019 | Amsterdam, Netherlands
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
Global Hematology 2019
4
th
International Conference on
HEMATOLOGY AND
BONE MARROW TRANSPLANTATION
EFFECT OF STEMREGENIN1 AND SB431542 SMALL MOLECULES ON EX VIVO
EXPANSION OF UMBILICAL CORD BLOOD HEMATOPOIETIC STEM CELLS ON
BIOCOMPATIBLE POLYETHER SULFONE NANOFIBER SCAFFOLDS
Sorush Niknamian
The Weston A Price Foundation, USA
C
ord blood hematopoietic stem cells (HSCs) with several advantages including low chance of viral contam-
ination and low rate of Graft versus host disease (GvHD) are appropriate candidate for vast medical appli-
cations such as transplantation. The main obstacle of cord blood HSCs is the low number cells. To improve ex
vivo expansion of umbilical cord HSCs author introduced a new culture system. Isolated HSCs were seeded in
three-dimensional (3D) on Polyethersulfone (PES) scaffolds and two-dimensional (2D) culture conditions and
treated with SB431542 and Stemregenin1 (SR1) small molecules. On the fifth and tenth days the expanded
cells in different groups were investigated for expression of specific markers by flow cytometry, expression
of some stemness genes by qRT-PCR and colony formation by methocult medium. SR1 molecule significantly
increased expansion of CD34+ cells while SB431542 induced more CD34+/38+ cells. Also SB431542 treated
cells showed higher colony formation capacity. SR1 increased the expression of c-Myc, HOXB4 and SALL4 while
SB431542 seemed to inhibit HOXB4 expression and increase SALL4.Together this study introduced a new ex
vivo culture setting for further medical application of HSCs. Their data showed simultaneous use of these two
small molecules can provide appropriate outcome for HSCs transplantation includes both of engraftment and
repopulation.
Hematol Blood Disord 2019, Volume 2