genetic-engineering-2019-keynote

4 / 5

allied
academies
Page 15
Journal of Research and Reports on Genetics | Volume 3
November 07-08, 2019 | Melbourne, Australia
Molecular Biology and Genetic Engineering
International Conference on
Notes:
Myelodysplastic syndrome (MDS) is the disorder
of hematopoietic stem cells. In MDS patients,
leukemia progression is also associated with iron overload.
Hydroxybutyrate dehydrogenase type 2 (BDH2) catalyzes
the production of 2,5-dihydroxybenzoic acid (DHBA), an
iron-binding component. In the present study, we assessed
whether BDH2 can serve as a predictor for leukemia
progression inMDS. The higher BDH2 expression (15%) group
showed a greater risk for leukemia progression than the
lower expression group (3.18%) (P=0.017). Additionally, we
investigated the mechanism underlying the prognostic ability
of BDH2 by using RNA interference–mediated-knockdown
of BDH2 (BDH2-KD) in the acute leukemia cell line, THP1.
Cell cycle analysis, surface markers, and special stain studies
indicatedthatBDH2-KD induceddifferentiationanddecreased
the growth rate of THP1 cells, which was associated with the
retardation of cell cycle. Under next-generation sequences
analysis, we also found some candidate genes involving iron
metabolism pathway contribute to leukemia transformation
in MDS patients. Our study provides a foundation for further
research on the role of BDH2 and iron metabolism in the
pathogenesis of MDS.
Speaker Biography
Wen-Chi Yang has completed her MD Ph.D. from Kaohsiung medical
University. She had 2 years postdoctoral studies from Harvard Medical
School during 2007 to 2009 and half year postdoctoral studies from
Massachusetts Institue of Technology after then. She is a hematology,
medical oncology and hospice care specialist in Taiwan. She is the attending
physician of EDA hospital. She is also the chief staff of hematology-oncology
division and Biobank of EDA hospital. She is an assistant professor in
Medical school, I-Shou University. She set up a molecular medicine lab in
Yuan’s general hospital. Her interests are in hematology, molecular biology
and stem cell biology field.
e:
Wen-Chi Yang
EDA Hospital, Taiwan
A novel predictor of leukemia transformation in Myelodysplastic
syndrome patients
Wen-Chi Yang, J Res Rep Genet 2019, Volume 3
DOI: 10.35841/2591-7986-C1-001