gastroenterologists-2018-scientifictracks

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academies

Nov 15-16, 2018 | Paris, France

Gastroenterology and Digestive Disorders

International Conference on

Journal of Gastroenterology and Digestive Diseases | Volume 3

Role of bile in adequacy of the data of experiments on the small intestine of rats

in vitro

and

in vivo

Olha V Storchylo

Odessa National Medical University, Russia

tudies of the functions of the Small Intestine in the

experiment occur on model systems in conditions in vitro, in

situ, and in vivo. The creation of model conditions presupposes

the presence of limitations on certain parameters: for example,

in conditions of Digestion and absorption in vitro, mediums that

are not adequate to the real composition of intestinal juice are

usually used. Therefore, in addition to Saliva, Pancreatic and

Gastric Juice, they do not contain bile. Bile is necessary for the

emulsification of food lipids, the detoxification of pathogenic

Microflora coming with a food, for the activation of Pancreatic

Lipase, for the transportation of lipids through the Intestinal

wall into the Blood and Lymph. Accordingly, the influence of Bile

on the fluidity of membranes of enterocytes and, as a result,

on the modification of their digestive and transport capabilities

should be expected. Therefore, in own experiments in vitro

and in vivo, we investigated the effect of bile on the activity

of hydrolysis systems of dimeric substrates of Protein and

Carbohydrate origin and transport of the resulting monomers

in the Small Intestine of rats. A significant multidirectional effect

of bile on the activity of both hydrolytic and transport systems

of enterocytes is shown.

Speaker Biography

Olha V Storchylo graduated Odessa State University (Ukraine) at biochemistry in 1983.

She completed her post-graduate in human and animal physiology and biochemistry

at the Institute of Physiology Pavlov named of Academy of Sciences of USSR in 1988

and joined the Human and Animal Physiology Department of Odessa State University

as an Assistant Professor. From 2008 until now, she is an Associate Professor of Medical

Chemistry Department of Odessa National Medical University. Fields of interests are

nutrition, digestion and absorption in the small intestine and effects of milk thistle

fruits on it, total body irradiation, nutrigenomics, radio pharmacology.