3 / 6
Page 11
Notes:
J Gastroenterol Dig Dis 2017 | Volume 2, Issue 3
allied
academies
World Gastroenterological &
Gastroenterology and Endoscopy
October 30-31, 2017 | Toronto, Canada
World Congress on
T
hymosin beta4 (Tβ4), an actin sequestering protein, is
involved intissuedevelopment and regeneration. It prevents
inflammation and fibrosis in several tissues. We investigated
the Hepatoprotective role of Tβ4 in chronic ethanol (EtOH)
and lipopolysaccharide (LPS)-induced liver injury as well as in
liver regeneration after partial hepatectomy in chronic EtOH
fed mice. We demonstrated that Tβ4 treatment prevented
EtOH and LPS-mediated oxidative stress by decreasing ROS
and lipid peroxidation; and increasing the antioxidants,
glutathione and manganese-dependent superoxide dismutase.
It also prevented the activation of nuclear factor KappaB by
blocking the phosphorylation of IκB, thereby prevented pro-
inflammatory cytokine production. Moreover, Tβ4 prevented
fibrogenesis by suppressing the epigenetic repressor,
methyl-CpG binding protein2 that coordinately reversed the
expression of peroxisome proliferator-activated receptor-γ
and down-regulated fibrogenic genes, platelet derived growth
factor β-receptor, α-smooth muscle actin, collagen1, and
fibronectin, resulting in reduced fibrosis. Tβ4 also promoted
liver regeneration after partial hepatectomy in EtOH-fed mice
by increasing hepatocyte regeneration markers, hepatocyte
growth factor and its receptor (c-Met) and α-fetoprotein, as
well as proliferation markers, proliferating cell nuclear antigen
and Ki-67. Our data suggest that Tβ4 has antioxidant, anti-
inflammatory, anti-fibrotic and regenerative potential during
alcoholic liver injury.
Speaker Biography
Raj Lakshman is currently the Director of Research Laboratories and the Chief of Lipid
Research at the VA Medical Center, Washington, D.C. He also has joint appointments
as a Professor in the Departments of Biochemistry & Molecular Medicine as well as
in the Department of Medicine at the George Washington University, Washington,
D.C. He directs studies in the areas of Alcoholism, Alcoholic Liver Disease, Oxidative
Stress, Coronary Artery Disease, Lipids & Lipoproteins, Metabolic & Genetic Obesity,
Hepatotoxins and Gene Regulation & Expression. He joined the National Institute of
Health, to work on Alcoholic Hyperlipidemia under the able guidance of Professors
Richard Veech and Nobel Laureate, Hans Krebs. In 1979, he received the prestigious
VA Research Career Scientist Award working in the field of Alcohol and Alcoholism at
the VA Medical Center, Washington, D.C. He was honored the “Washington Heart Ball”
Research Award in 1990 in the field of Hyperlipidemia.
e:
Raj.Lakshman@va.govRaj Lakshman
George Washington University, USA
Hepatoprotective role of thymosin β4 (Tβ4) in alcoholic liver disease