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Page 25
May 22-23, 2019 | Rome, Italy
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Euro Green Chemistry 2019 Journal of Industrial and Environmental Chemistry | ISSN: 2591-7331 | Volume 3
CHEMISTRY AND
EURO GREEN CHEMISTRY
9th International Conference on
Young Research Forum | Day 1
Rocco Cancelliere et al., J Ind Environ Chem 2019, Volume 3 | DOI: 10.4066/2591-7331-C2-011
BIOCHAR AS INNOVATIVE MATERIAL FOR DEVELOPMENT AN ELECTROCHEMICAL
PLATFORM
Rocco Cancelliere 1, K Carbone2, M Pagano3 and L Micheli1
1 Università di Roma Tor Vergata, Italy
2 CREA Centro di ricerca per la frutticoltura, Italy
3 CREA-IT Monterotondo, Italy
In the present study, an innovative way to use biochar from spent grain has been reported to underline the
possibility to reuse the industrial food waste. The beer brewing process is one of the most polluting industri-
al processes, generating a huge amount of wastewater effluent and solid wastes (i.e. spent grain and yeast).
Among them, spent grain can constitute as much as 85% of a brewery’s total by-products. As a consequence,
there is a great interest to find innovative ways to prevent spent grain from going to waste. At this regard,
Sperandio et al. developed a process for the production of biochar (charcoal) from dried spent grain through a
thermochemical process of pyro-gasification. Biochar is considered a good agricultural soil improver, with high
content of carbon and nitrogen able to promote water and nutrient retention, thus reducing the need of water
and chemical fertilizers. In the present study, author presented an innovative way to use biochar from spent
grain for the realization of screen printed electrodes, prepared with the modification of SPEs by drop casting
with a stable dispersion of biochar (Biochar/SPE sensor), have been reported. This study was conducted using
different electro-active species, such as ferricyanide, benzoquinone, epinephrine, ascorbic and uric acid in or-
der to understand the electrochemical behaviour of the modified electrode. The results were compared with
those of commercial screen-printed electrodes confirming that modification allowed obtaining a sensor with
improved electrochemical behaviour in terms of resolution, peak-to-peak separation, current intensity, and the
resistance of charge transfer. Tyrosinase biosensors (Ty/Biochar/SPE) has been developed using the Biochar/
SPE for the determination of epinephrine. The detection has been performed by measuring the current due to
the reduction of the corresponding quinone at low potential, equal to -0.310V for epinephrine. The experimen-
tal conditions for the tyrosinase immobilization and the analytical parameters such as applied potential and pH
of buffer have been studied and optimized. Under these conditions, the electrochemical biosensors have been
characterized. A linear working range of epinephrine was obtained from 0.05 up to 0.5mm. The detection limit
is 2×10-4 mm for developed biosensors. The biosensors construction was highly reproducible.
Rocco Cancelliere is a PhD student in Chemical Sciences and Technologies at the University of Rome Tor Vergata, Italy. He obtained
his Master’s Degree in Chemistry in the Department of Analytical Chemistry of the same University in 2018 where he also obtained
a Post graduate scholarship of three months before being eligible for the PhD program. In the last year, he got the opportunity to
work for the Center for Measurement and Information Systems (CEMIS) in Kajaani, Finland to work on the development of electro-
chemical devices for monitoring systems.
BIOGRAPHY