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N o v e m b e r 2 6 - 2 7 , 2 0 1 8 | M a d r i d , S p a i n
&
&
BIOTECHNOLOGY
Euro Congress on
GENOMICS AND MOLECULAR BIOLOGY
International Conference on
CANCER SCIENCE AND THERAPY
Global Congress on
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Euro Biotechnology 2018 & Genomics Congress 2018 & Cancer Congress 2018 Journal of RNA and Genomics | ISSN: 2591-7781 | Volume 14
Sanghamitra Das et al., J RNA Genomics 2018, Volume 14
THERAPEUTIC REFURBISHMENT OF
ARSENICOSIS IN TESTES OF SWISS ALBINO
MICE BY SELENIUM
Sanghamitra Das, Anirban Banerjee and Samar Kumar Saha
Visva Bharati Santiniketan, India
In the present experiment a pentavalent arsenic compound, sodium arsenate
was administered to mice to raise a model organism with arsenicosis fol-
lowing intraperitoneal administration of sodium arsenate at the dosages of
25 mg kg-1 body weight and 30 mg kg-1 body weight for a period of 45 days.
Upon this model organism of arsenocosis ameliorative potentiality of a sele-
nium compound, sodium selenite is evaluated. After thorough investigation
on the pathological conditions developed in the vital organ testis due to arse-
nic toxicity and the following conclusion can be drawn. Arsenic exposure is
detrimental to the biological system. It causes damage to the testis at tissue
level, cellular level which are ultimately reflected in physiological disruption.
Significant reductions in the number and diameter of seminiferous tubules as
well as in the number of tubules containing healthy germ cells indicate the ar-
senic induced gonadal pathology and oligospermia. After selenium treatment,
the ameliorative groups showed all the features of normozoospermia with
respect to structure, count and motility. Serum testosterone concentrations,
GSH and GST levels were also decreased in all successive treated groups and
these were refurbished by the treatment with sodium selenite. Apoptosis was
detected by chromatin condensation assay, ROS generation and FACs due to
sodium arsenate treatment while it was refurbished by sodium selenite. Our
fruitful observations support the notion that sodium arsenate impairs male
reproductive function by inducing oxidative stress and apoptosis, and these
experimental results suggest that selenium supplementation through oral
route improved sodium arsenate induced toxicity on testis.
Sanghamitra Das has completed his PhD in 2014 from
Visva Bharati Santiniketan, India. She is now engaged in
research in toxicology and vector borne diseases in IICB,
Kolkata and Visva Bharati Santiniketan and working in
Govt Health Department as District Entomologist.
BIOGRAPHY