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N o v e m b e r 2 6 - 2 7 , 2 0 1 8 | M a d r i d , S p a i n
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BIOTECHNOLOGY
Euro Congress on
GENOMICS AND MOLECULAR BIOLOGY
International Conference on
CANCER SCIENCE AND THERAPY
Global Congress on
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
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alliedacademies.comYEARS
Euro Biotechnology 2018 & Genomics Congress 2018 & Cancer Congress 2018
Journal of RNA and Genomics
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ISSN: 2591-7781
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Volume 14
Andrey Shirak et al., J RNA Genomics 2018, Volume 14
MAPPING OF SEX DETERMINATION
REGIONS IN CHITRALADA HYBRID STRAIN
TOWARDS ALL-MALE FRY PRODUCTION
Andrey Shirak
1
, Lior Dor
1
, Joel I Weller
1
, Micha Ron
1
Eyal Seroussi
1
, Tatyana Zak
2
and
Ayana Benet-Perlberg
2
1
Agriculture Volcani Centre, Israel
2
Dor Research Station, Israel
E
ffective farming of tilapia requires all-male culture, characterized by uni-
formity and high growth rate. This is currently achieved by larval mascu-
linization using steroid hormones or by crossbreeding
Oreochromis niloticus
females with
O. aureus
males. While the first technique has severe environ-
mental impacts and has been banned in Europe, the second has low yields
due to a reproductive barrier related to sexual behavior differences between
the parental species. Moreover, pure bred brood stocks are prone to contam-
ination by hybrids. Several breeding programs demonstrated higher growth
rates of crossed strains as compared to pure bred local varieties. However,
hybridization leads to segregation of a larger number of sex determining fac-
tors and thus complicates the genetic control of sex determination (SD). We
performed a genome wide association study by applying genotyping-by-se-
quencing technique for a selected full sub family of the Chitralada hybrid
strain (parents, 14 males and 79 females). The seven most significant as-
sociations based on 1,956 informative SNP markers were also validated by
analysis of microsatellite markers located in the relevant regions. Microsatel-
lite markers in linkage groups (LGs) seven and fifteen supported the associ-
ation of these newly discovered regions for SD. Together with the previously
described SD effect on LG23, these three loci provided good explanation for
SD in the analyzed tilapia family. Analysis of the effect of each of these loci
allowed constructing specific breeding scheme for all male fry production in
the Chitralada hybrid strain.
Andrey Shirak has his expertise in animal genetics; de-
tection and investigation of mechanisms underlying the
inheritance of categorical and quantitative traits; and
implementation of scientific finding in the improvement
of animal production. His hypothesis is that overcoming
the behavioral barrier between different tilapia species
is a key step for mass production of all-male tilapia,
through manipulation of sex determination by species
hybridization. Following 8 years of experimentation, his
hypotheses gain support from the actual determination
and localization of the genetic loci that are involved in
sex determination and in reproductive communication,
including the discovery of male-specific AMH duplica-
tion. The Chief Scientist (Israeli ministry of agriculture)
and ISF (Israeli Science Foundation) have supported the
present study over this period, allowing implementation
of knowledge from different disciplines of genomics, and
aquaculture in development of intensive technology for
tilapia production.
shiraka@agri.gov.ilBIOGRAPHY