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Ethnopharmacology 2019
& Physiotherapy Congress 2019
Asian Journal of Biomedical and Pharmaceutical Sciences
ISSN: 2249-622X | Volume 9
Page 46
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
March 27-28, 2019 | Amsterdam, Netherlands
&
PHARMACOLOGY AND ETHNOPHARMACOLOGY
7th International Conference and Exhibition on
5th GLOBAL PHYSIOTHERAPY, PHYSICAL
REHABILITATION AND SPORTS MEDICINE
Joint Event on
NEW CURCUMIN FORMULATION FOR THE TREATMENT OF CUTANEOUS
T-CELL LYMPHOMA
Antonios Trochopoulos1,3, Mirela Marinova1,3, Maya Zaharieva2 , Krassimira Yoncheva1,
Martin R Berger3 and Spiro Konstantinov 1
1 Medical University of Sofia, Bulgaria
2 Bulgarian Academy of Sciences, Bulgaria,
3 German Cancer Research Centre, Germany
C utaneous T-cell lymphomas (CTCL) are a group of heterogeneous life-threatening extranodal T-cell lymph-
oproliferative neoplasms, of which Mycosis Fungoides (MF) and Sézary syndrome (SéS) are the most prom-
inent subtypes. Taking into account the acquired resistance of malignancies in general, curcumin, a natural
pigment with proven antineoplastic effect and insignificant toxicity, could serve as a therapeutic agent in com-
bination regimes. Moreover, with inflammation playing a major role in the pathogenesis of CTCL, it is clear
that compounds with antineoplastic/anti-inflammatory activity like curcumin, are favorable. In this study, we
tested the antitumor efficacy of curcumin in ethanol solution, as well as incorporated into nanoparticles (mixed
micelles based on Pluronic®123 and Pluronic® 127). Both forms of curcumin were tested on 3 CTCL cell lines,
namely HuT-78, HH and MJ. MTT-dye reduction assay showed cytotoxic effects in all 3 CTCL cell lines for both
curcumin formulations with the IC50 values varying from 29.01 μM to 31.17 μM (ethanol solution) and 4.134
μΜ to 29.76 μM (nano-curcumin). The nano-formulation exerted faster cytotoxic effects (MTT-dye assay), which
can be explained by its faster internalization into the cells as measured by fluorescent microscopy and HPLC
analysis of the curcumin content in cell culture medium. Western blot analysis showed downregulation of im-
portant protein molecules regarding proliferation and survival such as: WT-1, ALK, p-JAK2, p-JAK3 for both
formulations. In conclusion, in this study we compared two different curcumin formulations and, as the active
ingredient is the same, we saw similar down-regulating effects on signal transduction proteins responsible for
proliferation and apoptosis escape. It can be assumed that the inclusion of curcumin into polymeric nanoparti-
cles will ameliorate its low bioavailability and support its faster internalization into the tumor cells.
Antonios Trochopoulos et al., Asian J Biomed Pharmaceut Sci 2019, Volume 9 | DOI: 10.4066/2249-622X-C1-018
Antonios Trochopoulos did master’s in pharmacy at Medical University of Sofia, Bulgaria. In 2016 he did his PhD in
department of pharmacology, toxicology, pharmacotherapy in Medical University of Sofia. In 2017-2018 he worked in
German Cancer Research Center (DKFZ),t Germany.
BIOGRAPHY