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Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4
allied
academies
November 02-03, 2017 | Chicago, USA
Embryology and In vitro Fertilization
World Congress on
T
his study was aimed to improve the efficiency of nuclear
reprogramming by treating embryos with HDACi. We
investigated the effects of two novel inhibitors, HDACi-14 and
-79, at the concentrations of 0, 1, 2, or 4μMon thedevelopment
of embryos cloned by the oocyte bisection cloning technique
(OBCT). Blastocyst rates of the reconstructed embryos reached
60% in the 2 μM HDACi-14-treated groups, which was higher
(p<0.05) compared to the untreated group (36.9%). Similarly,
HDACi-79 treatment at 2 and 4 μM also conferred higher
(p<0.05) blastocyst rates than that of the untreated group (79.4,
74.2, and 50.0%, respectively). Histone acetylation profile by
bothHDACi-14 (2 μM) and -79 (2 μM) treatments demonstrated
a drastic increase (p<0.05) mainly in two cell stage embryos
when compared to the control group. After seeding on the
feeder cells, the aggregated cloned blastocysts produced by the
HDACi-79 treatment showed a significant increase of primary
outgrowths compared to the control group (60.0% vs. 42.9%;
p<0.05). Finally, the cloned embryo-derived ES cell lines from
aggregated cloned embryos produced from the HDACi-79/14
treatment and the control groups were established. These
novel histone de-acetylation inhibitors can improve blastocyst
formation and increase the derivation efficiency of ntES cell
lines from the cloned porcine embryos produced
in vitro
.
Speaker Biography
Jyh-Cherng Ju earned his PhD training from Department of Animal Science, Cornell
University, New York, USA, in 1998, and then came back to Taiwan to work for
National Chung-Hsing University, Taichung, Taiwan. In 2012, he moved to Graduate
Institute of Biomedical Sciences, China Medical University (CMU) and the Hospital
CMUH, Taichung, Taiwan. He has been working on embryo/animal cloning by somatic
cell nuclear transfer, pluripotent stem cells, particularly in domestic species, and
thermal impacts on embryo development and germ layer differentiation. He has also
established a Core Lab for Stem Cell Research in CMUH to differentiate human, as
well as animal, ES cells into three germ layer cell lineages, such as motor neurons,
cardiomyocytes and hepatocytes from normal and disease individuals, for research
and therapeutic purposes.
e:
jcju@dragon.nchu.edu.twJyh-Cherng Ju
China Medical University and Hospital,Taichung, Taiwan
Histone de-acetylation inhibitors (HDACi) and embryo aggregation enhance
development and ntES cell derivation in cloned pig embryos