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Page 15
Res Rep Gynaecol Obstet 2017 | Volume 1 Issue 4
allied
academies
November 02-03, 2017 | Chicago, USA
Embryology and In vitro Fertilization
World Congress on
Objective: Preimplantation genetic screening (PGS) is
becoming more frequently employed for detection of
aneuploid embryos to prevent transmission of genetic
defects. A few cells are micro-surgically removed from the
embryo to analyze the DNA, thus selecting embryos with the
highest potential for implantation to optimize a woman’s
chance of healthy live birth and reduce the risk of miscarriage
due to chromosomal aneuploidy. The objective of this study
was to determine incidence of embryonic aneuploidy in
different age groups of Saudi patients undergoing ICSI-PGS
cycles at Thuriah Medical Center, Riyadh, Saudi Arabia.
Design: A retrospective study of patients undergoing ICSI-
PGS from Jan to Dec 2016 was done.
Materials & Methods: Data from 248 patients undergoing
ICSI-PGS were analyzed. The ovarian stimulation, oocyte
retrieval, ICSI, PGS, culture and transfer were performed
according to standard protocols. All sperm samples were
from male partners; 242 fresh ejaculates, four frozen-
thawed micro-TESE and two TESA. The embryo biopsies were
performed on day-3 and analyzed using fluorescence in-situ
hybridization with probes for chromosomes 13, 18, 21, X and
Y. The total embryos analyzed were 1055 and grouped into
normal, abnormal, mosaic and undiagnosed. The patients
were grouped into following age categories; <35, 35-37,
38-40, 41-42 and 43-48 yrs. The statistical analyses were
performed by SPSS.
Results: The percentages of euploid embryos were; 43,
46, 42, 24 and 28 in <35, 35-37, 38-40, 41-42 and 43-48
year females, respectively (Table 1). The percentages of
aneuploidy embryos were; 36, 32, 37, 53 and 54 in these
study groups, respectively. The percentages of normal
embryos decreased and percentages of abnormal embryos
increased significantly in females >41 yrs of age. The
percentage of mosaic embryos was significantly higher in
43-48 year females. The pregnancy rate was 28, 33, 22, 0
and 25 % in <35, 35-37, 38-40, 41-42 and 43-48 years age
groups, respectively. In an earlier study of Saudi population
conducted in 2013, the abnormal embryos constituted 36 %
in women with an average age of 34.9 yrs. In our study the
% of abnormal embryos in <35 years age group is similar;
however, it increased significantly in women ≥41 yrs. Such
data is not available in the previous report.
Conclusions: The embryonic aneuploidy rate is similar until
age 40 years, however, it increases significantly in embryos
of 41 years or older women.
Speaker Biography
Dr. Javed is Director of ART Laboratories at Thuriah Medical Center, Riyadh, Saudi
Arabia. He has been certified by Canadian Fertility and Andrology Society. He is
member of Practice Committee and Chair of Certification Committee of American
College of Embryology, USA. He is member of many professional societies including
American Society for Reproductive Medicine, Canadian Fertility and Andrology Society
and European society for Human Reproduction and Embryology. His professional
carrier started after obtaining Doctor of Veterinary Medicine Degree in 1981. He
earned MS in Reproductive Physiology in 1984. His initial research experiments were
in Embryo Physiology of research animals. This was the time when this technology
was just beginning in human. In 1986, his curiosity for further knowledge, took
him to Washington State University, USA for PhD in Embryo Physiology. He earned
PhD in 1990. He had the opportunity to earn 2 post doctorate fellowships; first at
Kyoto University, Japan and second at University of Georgia, Athens, USA. At these
institutions, he conducted research on in vitro fertilization, embryo culture and embryo
vitrification. In 1997, he joined Toronto Institute for Reproductive Medicine in Toronto,
Canada as Laboratory Manager and served for more than 4 years. In 2001, he was
selected by University of Michigan, USA as Senior Clinical Technologist to initiate sperm
cryopreservation facility for cancer patients. He was then selected by Toronto Centre
for Advanced Reproductive Technology as Clinical Embryologist where he worked
for 8 years. In 2010, he took the responsibility of Director ART Laboratories at Astra
Fertility Clinic which had 4 locations across Greater Toronto Area. In Oct 2015, he was
selected as Director ART laboratories at Thuriah Medical Center, Saudi Arabia. Dr. Javed
has extensive research and clinical experience in all aspects of Assisted Reproductive
Technology. He has published extensively and has written a book chapter. He
implements all measures for best care of sperm, eggs and embryos in the laboratory to
achieve high levels of pregnancy.
e:
Murid Javed
Thuriah Medical Center, Saudi Arabia
Incidence of embryonic aneuploidy in different age groups of Saudi population