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Joint Event
November 29-30, 2019 | Frankfurt, Germany
28
th
International Conference on
3
rd
International Conference on
Diabetes and Endocrinology
Diabetes and Metabolism
&
2
0
1
9
CONGRESS
DIABETES
2019
DIABETES
Journal of Diabetology | Volume 3
The effect of Sticopus hermanii- Hyperbaric Oxygen Therapy to osteogenesis of
diabetic periodontitis
Dian Mulawarmanti
and
Widyastuti Widyastuti
Universitas Hang Tuah, Indonesia
Background:
Diabetes and periodontitis affecting a large
number of populations worldwide. Osteoprogeterin (OPG) is
cytokine regulating osteoclastogenesis, related to osteoclast
and osteoblast implicated in various inflammations including
periodontitis and diabetes. Hyperbaric oxygen therapy
(HBOT) has been used as adjuvant therapy to heal chronic
wound problem. Sticopus Hermanii (SH) has been consumed
as food rich with content of sulfated glycosaminoglycans
(GAGs) which affect wound healing. The aim of this study is
to examine the effect of HBOT and Sticopus hermanii to the
exression of OPG, osteoclast and osteoblast in periodontitis
diabetic rats.
Methods and Material:
The reasearch was an experimental
laboratories with post test only control group design. Fourty
five maleWistar rats aged 8-10 weeks were equally divided in
5 groups of: healthy group (G1), diabetic periodontitis group
(G2), diabetic periodontitis group treated SH (G3), diabetic
periodontitis group treated with HBOT 2,4 ATA 3x30’ interval
5’ for 7 days (G4), diabetic periodontitis group treated with
combination SH-HBOT (G5). Diabetes was conducted by
single dose of streptozotocin 65 mg/kg of BW intraperitoneal,
while periodontitis were induced by Porphyromonas
gingivalis ATCC 33277. The OPG expressions were examined
by immunohistochemistry, while the number of osteoclast
and osteoblast by hematoxylin eosin staining.
Results:
It was found that combination SH-HBOT (G5) in OPG
could increased (12.50 ± 2.082) than diabetic-periodontitis
group(G2) 2.50 ± 0.577. The combination SH-HBOT (G5) in
osteoblast can increased significantly (28.47 ± 3.20) than
diabetic-periodontitis group(G2) 9.22 ± 4.95. Osteoclast was
decreased significantly in combination SH-HBOT (G5) 3.50 ±
0.957 than diabetic-periodontitis group diabetic-periodontitis
group 0.500 ± 816. (p<0.05)
Conclusion:
HBOT increased OPG expression and
osteoblast,reduced the number of osteoclast in periodontitis
diabetic rats.
Speaker Biography
Dian Mulawarmanti has completed her PhDs from Airlangga University,
Indonesia. She is associate professor of Universitas Hang Tuah Indonesia
as a lecturer in faculty of dentistry. She got some national research grants
fromDirectorate of Higher Education of IndonesoianMinistry of Education
& Culture about Marine Biota and HBOT Research in Dentistry since 2013
–at present.
e:
dian.mulawarmanti@hangtuah.ac.id