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September 09-10, 2019 | Edinburgh, Scotland

Global Summit on

International Conference on

Dermatology and Cosmetology

Wound Care, Tissue Repair and Regenerative Medicine

Joint Event

Journal of Dermatology Research and Skin Care | Volume 3

Dermatol Res Skin Care, Volume 3

Activation of p38, p21, and NRF-2 mediates decreased proliferation of human dental pulp

stem cells cultured under 21% O2

Marya El Alami

University of Valencia, Spain

igh rates of stem cell proliferation are important in

regenerative medicine and in stem cell banking for

clinical use. Ambient oxygen tensions (21% O2) are normally

used for in vitro culture, but physiological levels in vivo range

between 3% and 6% O2. We compared proliferation of

human dental pulp stem cells (hDPSCs) cultured under 21%

versus3%O2. The rate of hDPSC proliferation is significantly

lower at 21% O2 compared to physiological oxygen levels

due to enhanced oxidative stress. Under 21% O2, increased

p38 phosphorylation led to activation of p21. Increased

generation of reactive oxygen species and p21 led to

activation of the NRF-2 signaling pathway. The upregulation

of NRF-2 antioxidant defense genes under 21% O2 may

interact with cell-cycle-related proteins involved in regulating

cell proliferation. Activation of p38/p21/NRF-2 in hDPSCs

cultured under ambient oxygen tension inhibits stem cell

proliferation and upregulates NRF-2 antioxidant defenses.

Speaker Biography

Marya El Alami has completed her PhD at the age of 30 years from

University of Valencia, Spain. She has 9 publications. She is a doctor of

pharmacy and has a master on pharmacology. She speaks 5 languages.