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S e p t e m b e r 0 3 - 0 4 , 2 0 1 8 | B a n g k o k , T h a i l a n d
allied
academies
Joint Event on
Dental Congress 2018 & World Dermatology 2018
Archives of General Internal Medicine
|
ISSN: 2591-7951
|
Volume 2
WORLD DERMATOLOGY AND COSMETOLOGY CONGRESS
DENTISTRY AND ORAL HEALTH
&
International Conference on
Arch Gen Intern Med 2018, Volume 2 | DOI: 10.4066/2591-7951-C2-006
THE OPTIMAL TIMING OF ADIPOSE DERIVED STEM CELLS INJECTION TO
IMPROVE SKIN FLAP SURVIVAL IN A RAT MODEL
Ahmed Atef
Ain Shams University, Egypt
Background:
Skin flap surgery is a cornerstone in plastic surgery. However, flaps may encounter postoperative partial or complete
necrosis. Different modalities have been used to enhance skin flap survival. Adipose-derived stem cells (ASCs) can promote
angiogenesis and improve skin flap survival. We aimed to investigate the appropriate timing of ASC administration to get the
maximal angiogenic effect and better skin flap survival in rat model.
Methods:
Thirty-two male albino Wistar rats were used to study the role of ASCs on caudally based (2 × 8 cm) random pattern
skin flap survival; the 32 male albino rats were equally divided into four groups according to timing of ASC injection. We applied
the ASCs at three different timing, group A (local injection of ASCs along the flap axis simultaneously with flap elevation), group B
(2 days preoperatively), and group C (7 days preoperatively). In the control group (group D), the flap was injected with phosphate
buffered saline at the time of flap elevation. Skin flap survival was evaluated on the seventh postoperative day through percentage
of flap survival, capillary density, and vascular endothelial growth factor (VEGF) expression.
Results:
ASCs led to a statistically significant increase in skin flap viability when administered simultaneously with flap elevation or
2 days prior to flap elevation. This went along with significant increase in capillary density and VEGF expression. However, the ASCs
had no effect on flap survival when injected 1 week prior to flap elevation.
Conclusion:
We demonstrate that the skin flap viability can be enhanced by local ASC administration simultaneously with flap
elevation or 2 days prior to flap elevation. Their beneficial effect is attributed to paracrine secretion VEGF which enhanced the skin
flap vascularity. The angiogenic effect of ASCs can be maximized when the ASCs are injected at the time of flap elevation.