dental-congress-2019-scientifictracks-abstracts

Note:

Dental Congress 2019

Journal of Clinical Dentistry and Oral Health | Volume 3

Page 38

OF EXCELLENCE

IN INTERNATIONAL

MEETINGS

YEARS

April 15-16, 2019 | Milan, Italy

DENTISTRY

AND ORAL HEALTH

International Conference on

ARE E-CIGS SAFE? AN EVALUATION OF CYTOTOXIC AND CYTOGENETIC DAMAGE

IN BUCCAL MUCOSA

Janete Dias Almeida, Lígia Ângelo Tavares Schwarzmeier, Beatriz Serralheiro da Cruz, Camila

Cristina Panisello Ferreira, Bruna Fernandes do Carmo Carvalho, Mônica Ghislaine Oliveira Alves,

Celina Faig Lima Carta and Jaqueline Ribeiro Scholz

São Paulo State University, Brazil

lectronic cigarettes also known as e-cigarettes, e-vaporizers or electronic nicotine delivery systems are de-

vices that promote the vaporization of nicotine without the burning of tobacco. The aim of this study was to

investigate cytogenetic and cytotoxic damage through the evaluation of micronuclei frequency and metanu-

clear anomalies in the oral mucosa of users of electronic cigarette users compared to smokers of conventional

cigarettes, ex-smokers and non-smokers. The patients were divided into four groups: control; e-cig; smokers

and ex-smokers. The samples were collected by means of exfoliative cytology of the lateral region of the tongue

and floor of the mouth. The smears obtained were fixed and the slices stained by the Feulgen method for in-

vestigation of micronuclei and metanuclear anomalies. A significant difference was observed only between

the smoking and control groups in the frequency of micronuclei. As for metanuclear anomalies, comparisons

in karyolysis were significant between: smokers and control, e-cig and control, as well as e-cig and ex-smokers;

karyorrhexis: only between smoker and control groups; binucleation: between e-cig and ex-smoker groups

and e-cig and control groups; broken eggs: between e-cig and all other groups; nuclear bud: between e-cig

and ex-smokers, as well as e-cig and control. e-cig users, most ex-smokers of conventional cigarettes and alco-

hol users, presented genotoxicity and cytotoxicity in the cells of the oral mucosa.

Janete Dias Almeida et al., J Clin Dentistry Oral Health 2019, Volume 3

Janete Dias Almeida has completed her PhD at the age of 33 years from São Paulo State University, Brazil. She is Full time Professor at

Institute of Science and Technology, São José dos Campos, Department of Biosciences and Diagnosis, São José dos Campos, Brazil.

She has published papers on her research in reputed journals.

BIOGRAPHY