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Case Reports in Surgery and Invasive Procedures | Volume 3
March 11-12, 2019 | London, UK
Biomarkers
Plastic and Cosmetic Surgery
International Conference on
International Conference on
Joint Event
&
Noninvasive protein biomarkers for detection of transplant injury in kidney transplantation
Tara Sigdel
University of California San Francisco, USA
Background
: The human urinary proteome provides an
assessment of kidney injury with specific biomarkers for
different kidney injury phenotypes.
Method
: In an effort to fully map and decipher changes in the
urine proteome and peptidome after kidney transplantation,
renal allograft biopsy matched urine samples were collected
from 396 kidney transplant recipients. Centralized and blinded
histology data from paired graft biopsies was used to classify
urine samples into diagnostic categories of acute rejection,
chronic allograft nephropathy, BK virus nephritis, and stable
graft. Two hundred forty-five urine samples were analyzed by
liquid chromatography-mass spectrometry using isobaric Tags
for Relative and Absolute Quantitation (iTRAQ) reagents. From
a group of over 900 proteins identified in transplant injury, a set
of 131 peptides were assessed by selected reaction monitoring
for their significance in accurately segregating organ injury
causation and pathology in an independent cohort of 151
urine samples. Ultimately, a minimal set of 35 proteins were
identified for their ability to segregate the 3 major transplant
injury clinical groups.
Results
: Our analysis identified a panel of 11 urinary peptides
for acute rejection (93% area under the curve [AUC]), 12 urinary
peptides for chronic allograft nephropathy (99% AUC), and 12
urinary peptides for BK virus nephritis (83% AUC).
Conclusion
: Urinaryproteomediscoveryand targetedvalidation
can identify urine protein panels for rapid and noninvasive
differentiation of different causes of kidney transplant injury,
without the requirement of an invasive biopsy.
e:
tara.sigdel@ucsf.edu